This contribution renders an affordable clean method for deposition of Ag, ZnO and ZnO/Ag nanoparticles on polyester (PET) substrates by in situ wet chemical approach utilizing available Seidlitzia rosmarinuser ashes. Replacement of using hazardous chemicals such as toxic reductants and solvents as well as simplification of the process for industry through one-step process instead of separate synthesis/buying nanoparticles are the advantages of this route from the both human health/environmental and economic observations. Successful formation of silver, zinc oxide and silver/zinc oxide nanoparticles on PET fabrics was evidenced by field-emission scanning electron microscope, mapping images and energy-dispersive x-ray spectroscopy. The nano-treated samples including PET/ZnO, PET/Ag and PET/Ag/ZnO have proper bactericidal performances of 71, 94, 99 % toward Staphylococcus aureus and 63, 91 and 93 % against Escherichia coli bacteria, respectively. Also, the color difference among the prepared samples and the raw PET fabric is very slight which is preferred for textile and garment industry. Moreover, tensile strength of the modified fabrics was slightly promoted compared to the raw PET sample owing to nanoparticles formation over the fabric surface and the modified fabrics indicated softer handle and greater flexibility. In addition, wettability behavior for all the modified samples together with resistance to ignition in the case of ZnO deposition on the fabric was promoted. Overall, the introduced technique in this research can contribute to textile industry for producing antibacterial textiles with good textile performance using a clean way through formation of nanoparticles.