The zebrafish CreZoo: an easy-to-handle database for novel CreER(T2)-driver lines

Publikation: Beitrag in FachzeitschriftForschungsartikelBeigetragenBegutachtung

Abstract

We report a new open access database, the zebrafish CreZoo ( http://crezoo.crt-dresden.de ), which contains novel CreER(T2)-driver lines that express Cre fused to the mutated human ligand-binding domain of the estrogen receptor (CreER(T2)) in several tissues. Recently, the conditional Cre/loxP technology has been added to the toolbox for the precise manipulation of the zebrafish genome, but currently the number of CreER(T2)-driver lines is limited. To enlarge the pool of existing CreER(T2)-driver lines, we conducted a genome-wide screen using a gene trap cassette comprising a splice acceptor and an mCherry-tagged variant of CreER(T2). All molecular and expression data obtained in this screen are summarized in the CreZoo database, which currently comprises an inventory of about 47 Cre-driver lines expressing CreER(T2) in a cell- and tissue-specific manner during development and adulthood. Combined with other Cre-dependent effector lines, the CreZoo will be a great tool to manipulate the zebrafish genome.

Details

OriginalspracheEnglisch
Seiten (von - bis)259-63
Seitenumfang5
FachzeitschriftZebrafish
Jahrgang10
Ausgabenummer3
PublikationsstatusVeröffentlicht - Sept. 2013
Peer-Review-StatusJa

Externe IDs

researchoutputwizard legacy.publication#54010
PubMed 23668932
Scopus 84883316426
ORCID /0000-0003-0283-0211/work/142257350

Schlagworte

Schlagwörter

  • Animals, Databases as Topic, Zebrafish