The fission yeast rad22 gene, having a function in mating-type switching and repair of DNA damages, encodes a protein homolog to Rad52 of Saccharomyces cerevisiae

Publikation: Beitrag in FachzeitschriftForschungsartikelBeigetragenBegutachtung

Beitragende

Abstract

The gene rad22 of the fission yeast Schizosaccharomyces pombe has a function in DNA repair and mating-type switching. We have cloned the rad22 gene from a genomic gene bank by functional complementation of the switching defect. An open reading frame coding for a putative protein of 469 amino acids was found by sequence analyses. The rad22 gene contains no intron. A region of 126 amino acids in the N-terminal half of the Rad22 protein has significant homologies (56% identity and 36% similarity) to the Rad52 protein of Saccharomyces cerevisiae. A rad22 disruption strain was constructed which seems to be inviable in a homothallic background. Southern blot analyses have shown that the rad22-67 mutant frequently gives rise to deletions in the mating-type region. These data indicate that the Rad22 protein has a function in the repair of DNA double-strand breaks.

Details

OriginalspracheEnglisch
Seiten (von - bis)5940-5944
Seitenumfang5
FachzeitschriftNucleic acids research
Jahrgang21
Ausgabenummer25
PublikationsstatusVeröffentlicht - 25 Dez. 1993
Peer-Review-StatusJa

Externe IDs

PubMedCentral PMC310478
Scopus 0027751660

Schlagworte

Schlagwörter

  • Amino Acid Sequence, Blotting, Southern, Cloning, Molecular, DNA Damage, DNA Repair/genetics, DNA, Fungal/genetics, DNA-Binding Proteins/genetics, Fungal Proteins/genetics, Genes, Fungal, Genes, Mating Type, Fungal, Genes, Switch, Molecular Sequence Data, Rad52 DNA Repair and Recombination Protein, Restriction Mapping, Saccharomyces cerevisiae/genetics, Saccharomyces cerevisiae Proteins, Schizosaccharomyces/genetics, Schizosaccharomyces pombe Proteins, Sequence Homology, Amino Acid

Bibliotheksschlagworte