Sulfated Hyaluronan Influences the Formation of Artificial Extracellular Matrices and the Adhesion of Osteogenic Cells

Publikation: Beitrag in FachzeitschriftForschungsartikelBeigetragenBegutachtung

Beitragende

Abstract

The aim of this study is to compare differentially sulfated hyaluronan (sHA) derivatives and chondroitin sulfate (CS) with respect to their ability to influence the formation of artificial extracellular matrices (aECMs) during in vitro-fibrillogenesis of collagen type I at high- and low-ionic strength. Analysis is performed using turbidity, biochemical assays, atomic force (AFM), and transmission electron microscopy (TEM). In general, high-sulfated glycosaminoglycans (GAGs) associate to a higher amount with collagen than the low-sulfated ones. The addition of GAGs prior to fibrillogenesis at low-ionic strength results in a dose-dependent decrease in fibril diameter. At high-ionic strength these effects are only obtained for the sHA derivatives but not for CS. Likewise, increasing concentrations and degree of GAG sulfation strongly affected the kinetics of fibrillogenesis. The impact of sulfation degree on F-actin location and fiber formation in SaOS-2 cells implies that adhesion-related intracellular signaling is influenced to a variable extent.

Details

OriginalspracheEnglisch
Seiten (von - bis)1783-1794
Seitenumfang12
FachzeitschriftMacromolecular Bioscience
Jahrgang14
Ausgabenummer12
PublikationsstatusVeröffentlicht - Dez. 2014
Peer-Review-StatusJa

Externe IDs

researchoutputwizard legacy.publication#61431
Scopus 84916242128
researchoutputwizard legacy.publication#84898
WOS 000346069600013
PubMed 25219504
ORCID /0000-0003-0423-4093/work/142239577
ORCID /0000-0002-5611-9903/work/142244047

Schlagworte

Forschungsprofillinien der TU Dresden

Fächergruppen, Lehr- und Forschungsbereiche, Fachgebiete nach Destatis

Schlagwörter

  • Atomic force microscopy (AFM), Chondroitin sulfate, Collagen fibrillogenesis, Hyaluronan, Matrix, extracellular matrices, osteogenic cells