Studying Complexes Between PPI Dendrimers and Mant-ATP

Publikation: Beitrag in FachzeitschriftForschungsartikelBeigetragenBegutachtung

Beitragende

  • A. Szulc - , Lodz University of Technology (Autor:in)
  • D. Appelhans - , Leibniz-Institut für Polymerforschung Dresden (Autor:in)
  • B. Voit - , Leibniz-Institut für Polymerforschung Dresden (Autor:in)
  • M. Bryszewska - , Lodz University of Technology (Autor:in)
  • B. Klajnert - , Lodz University of Technology (Autor:in)

Abstract

The aim of this study was to investigate the interactions between poly(propylene imine) (PPI) dendrimers and 2'-/3'-O-(N'-methylanthraniloyl)-ATP (Mant-ATP). Mant-ATP was used as a model molecule. Purine and pyrimidine nucleoside analogues are antimetabolites commonly used in therapy for cancer. Drug molecules can complex with dendrimers in two ways: therapeutic agents may be attached in dendrimer interior or bind to functional groups on the surface. Drugs attached to nanoparticles are characterized by improved solubility, pharmacokinetics and stability. Here, we have used poly(propylene imine) dendrimers of the 4th and 5th generations (PPI G4 and PPI G5) with primary amino surface groups partially modified with maltose (PPI-m) or without modification (PPI). We assessed the efficiency of complex formation in relation to dendrimer generation, pH of solution and the type of dendrimer used. A double fluorimetric titration method was used to estimate the binding constant (K (b) ) and the number of binding centers per molecule of the binding agent (n).

Details

OriginalspracheEnglisch
Seiten (von - bis)349-356
Seitenumfang8
Fachzeitschrift Journal of fluorescence
Jahrgang23
Ausgabenummer2
PublikationsstatusVeröffentlicht - März 2013
Peer-Review-StatusJa
Extern publiziertJa

Externe IDs

PubMed 23306952
Scopus 84879694282
ORCID /0000-0002-4531-691X/work/148607889

Schlagworte

Ziele für nachhaltige Entwicklung

Schlagwörter

  • Complexation, Dendrimer, Fluorimetric titration, Mant-ATP, Nucleoside analogues