Single-shot impulsive stimulated Brillouin microscopy by tailored ultrashort pulses
Publikation: Beitrag in Fachzeitschrift › Forschungsartikel › Beigetragen › Begutachtung
Beitragende
Abstract
Brillouin microscopy has become an important tool for investi-
gating the mechanical properties of tissue. The recently devel-
oped Impulsive stimulated Brillouin Scattering (ISBS) promises
a label-free, non-invasive measurements of viscoelastic proper-
ties of transparent samples and offers the potential for a high
temporal resolution. However, the spatial resolution of ISBS is
currently limited, which hinders its transfer to real-world appli-
cations. Increasing the spatial resolution of ISBS leads to an in-
crease in the energy density of the pump beams, which requires
a balancing of the excitation parameters to stay below the photo-
toxic threshold. This paper focuses on the influences of different
excitation parameters on the spatial, temporal and spectral res-
olution and their optimal values. Combined with the adoption
of a noise suppressing window function, a measurement rate of
20μs/pixel in hydrogel is achieved, which is promising for fast
3D imaging. The presented advanced impulsive stimulated Bril-
louin microscopy can be applied for fast tissue elastography to-
ward disease studies.
gating the mechanical properties of tissue. The recently devel-
oped Impulsive stimulated Brillouin Scattering (ISBS) promises
a label-free, non-invasive measurements of viscoelastic proper-
ties of transparent samples and offers the potential for a high
temporal resolution. However, the spatial resolution of ISBS is
currently limited, which hinders its transfer to real-world appli-
cations. Increasing the spatial resolution of ISBS leads to an in-
crease in the energy density of the pump beams, which requires
a balancing of the excitation parameters to stay below the photo-
toxic threshold. This paper focuses on the influences of different
excitation parameters on the spatial, temporal and spectral res-
olution and their optimal values. Combined with the adoption
of a noise suppressing window function, a measurement rate of
20μs/pixel in hydrogel is achieved, which is promising for fast
3D imaging. The presented advanced impulsive stimulated Bril-
louin microscopy can be applied for fast tissue elastography to-
ward disease studies.
Details
| Originalsprache | Englisch |
|---|---|
| Seiten (von - bis) | 1-9 |
| Seitenumfang | 9 |
| Fachzeitschrift | Journal of the European Optical Society-Rapid Publications |
| Jahrgang | 21 |
| Ausgabenummer | 1 |
| Publikationsstatus | Veröffentlicht - 14 Feb. 2025 |
| Peer-Review-Status | Ja |
Externe IDs
| Mendeley | 5c61f169-d097-3071-bc8f-9591f9e87534 |
|---|