Role of EPS in mitigation of plant abiotic stress: The case of Methylobacterium extorquens PA1

Publikation: Beitrag in FachzeitschriftForschungsartikelBeigetragenBegutachtung

Beitragende

  • Adele Vanacore - , Università degli Studi di Napoli Federico II (Autor:in)
  • Maria Concetta Forgione - , Università degli Studi di Napoli Federico II (Autor:in)
  • Domenico Cavasso - , Università degli Studi di Napoli Federico II (Autor:in)
  • Ha Ngoc Anh Nguyen - , Bottom-up Synthetic Biology (NFoG) (Autor:in)
  • Antonio Molinaro - , Università degli Studi di Napoli Federico II (Autor:in)
  • James P Saenz - , Bottom-up Synthetic Biology (NFoG) (Autor:in)
  • Gerardino D'Errico - , Università degli Studi di Napoli Federico II (Autor:in)
  • Luigi Paduano - , Università degli Studi di Napoli Federico II (Autor:in)
  • Roberta Marchetti - , Università degli Studi di Napoli Federico II (Autor:in)
  • Alba Silipo - , Università degli Studi di Napoli Federico II (Autor:in)

Abstract

Methylobacterium extorquens is a facultative methylotrophic Gram-negative bacterium, often associated with plants, that exhibits a unique ability to grow in the presence of high methanol concentrations, which serves as a single carbon energy source. We found that M. extorquens strain PA1 secretes a mixture of different exopolysaccharides (EPSs) when grown in reference medium or in presence of methanol, that induces the secretion of a peculiar and heterogenous mixture of EPSs, with different structure, composition, repeating units, bulk and a variable degree of methylation. These factors influenced 3D structure and supramolecular assets, diffusion properties and hydrodynamic radius, and likely contribute to increase methanol tolerance and cell stability. No direct methanol involvement in the EPSs solvation shell was detected, indicating that the polymer exposure to methanol is water mediated. The presence of methanol induces no changes in size and shape of the polymer chains, highlighting how water-methanol mixtures are a good solvent for refEPS and metEPS.

Details

OriginalspracheEnglisch
Aufsatznummer119863
FachzeitschriftCarbohydrate polymers
Jahrgang295
PublikationsstatusVeröffentlicht - 1 Nov. 2022
Peer-Review-StatusJa

Externe IDs

Scopus 85134534124
unpaywall 10.1016/j.carbpol.2022.119863
ORCID /0000-0001-8901-4377/work/142232417

Schlagworte

Schlagwörter

  • Abiotic stress, Conformation, Exopolysaccharide, Methylobacterium extorquens, Molecular modelling, NMR spectroscopy