Repellent guidance of regenerating optic axons by chondroitin sulfate glycosaminoglycans in zebrafish
Publikation: Beitrag in Fachzeitschrift › Forschungsartikel › Beigetragen › Begutachtung
Beitragende
Abstract
We analyzed the role of chondroitin sulfate (CS) glycosaminoglycans, putative inhibitors of axonal regeneration in mammals, in the regenerating visual pathway of adult zebrafish. In the adult, CS immunoreactivity was not detectable before or after an optic nerve crush in the optic nerve and tract but was constitutively present in developing and adult nonretinorecipient pretectal brain nuclei, where CSs may form a boundary preventing regenerating optic fibers from growing into these inappropriate locations. Enzymatic removal of CSs by chondroitinase ABC after optic nerve crush significantly increased the number of animals showing erroneous growth of optic axons into the nonretinorecipient magnocellular superficial/posterior pretectal nucleus (83% vs 42% in controls). In vitro, a substrate border of CSs, but not heparan sulfates, strongly repelled regenerating retinal axons from adult zebrafish. We conclude that CSs contribute to repellent axon guidance during regeneration of the optic projection in zebrafish.
Details
Originalsprache | Englisch |
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Seiten (von - bis) | 842-853 |
Seitenumfang | 12 |
Fachzeitschrift | Journal of Neuroscience |
Jahrgang | 22 |
Ausgabenummer | 3 |
Publikationsstatus | Veröffentlicht - 1 Feb. 2002 |
Peer-Review-Status | Ja |
Extern publiziert | Ja |
Externe IDs
PubMed | 11826114 |
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Schlagworte
ASJC Scopus Sachgebiete
Schlagwörter
- Chondroitin sulfate proteoglycans, Chondroitinase ABC, CNS regeneration, Extracellular matrix, Heparan sulfate, Neurite outgrowth, Retinal ganglion cell axons, Tenascin-R