RecE/RecT and Redα/Redβ initiate double-stranded break repair by specifically interacting with their respective partners
Publikation: Beitrag in Fachzeitschrift › Forschungsartikel › Beigetragen › Begutachtung
Beitragende
Abstract
The initial steps of double-stranded break (DSB) repair by homologous recombination mediated by the 5'-3' exonuclease/annealing protein pairs, RecE/RecT and Redα/Redβ, were analyzed. Recombination was RecA-independent and required the expression of both components of an orthologous pair, even when the need for exonuclease activity was removed by use of preresected substrates. The required orthologous function correlated with a specific protein-protein interaction, and recombination was favored by overexpression of the annealing protein with respect to the exonuclease. The need for both components of an orthologous pair was observed regardless of whether recombination proceeded via a single-strand annealing or a putative strand invasion mechanism. The DSB repair reactions studied here are reminiscent of the RecBCD/RecA reaction and suggest a general mechanism that is likely to be relevant to other systems, including RAD52 mediated recombination.
Details
Originalsprache | Englisch |
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Seiten (von - bis) | 1971-1982 |
Seitenumfang | 12 |
Fachzeitschrift | Genes and Development |
Jahrgang | 14 |
Ausgabenummer | 15 |
Publikationsstatus | Veröffentlicht - 2000 |
Peer-Review-Status | Ja |
Externe IDs
PubMed | 10921910 |
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ORCID | /0000-0002-4754-1707/work/142248116 |
Schlagworte
ASJC Scopus Sachgebiete
Schlagwörter
- DSB repair, Homologous recombination, RecE/RecT, Redα/Redβ, Specific interaction