Reactive Maleimido Dextran Thin Films for Cysteine-Containing Surfaces Adsorbing BSA

Publikation: Beitrag in FachzeitschriftForschungsartikelBeigetragenBegutachtung

Beitragende

  • Thomas Elschner - , University of Maribor (Autor:in)
  • Franziska Obst - , Friedrich-Schiller-Universität Jena (Autor:in)
  • Thomas Heinze - , Friedrich-Schiller-Universität Jena (Autor:in)
  • Rupert Kargl - , University of Maribor (Autor:in)
  • Karin Stana Kleinschek - , University of Maribor (Autor:in)

Abstract

The modification of surfaces by functional coatings or postmodification of films to control the adsorption of bovine serum albumin (BSA) is carried out. Surface functionalization with maleimido groups is achieved by spin coating of dextran derivatives. The layers are stabilized on the support material during thermal treatment or cross-linking. The thin films are characterized by atomic force microscopy, quartz crystal microbalance, FTIR spectroscopy, and X-ray photoelectron spectroscopy. Surface morphology, hydrophobicity, swelling behavior, and reactivity of dextran furfuryl carbamate bismaleimide adduct are suitable for the preparation of surfaces containing cysteine by thiol-ene reaction. The different functional surfaces obtained are investigated regarding their BSA adsorption behavior. Up to 9 mg m(-2) of BSA are adsorbed on the nanorough, hydrophilized films at different pH values (4, 6, and 8.5) and thus a blocking layer can be obtained. The BSA layer may resist nonspecific adsorption of biological molecules and can be interesting for prevention of biofilm formation on medical devices.

Details

OriginalspracheEnglisch
Aufsatznummer1600535
Seitenumfang8
FachzeitschriftMacromolecular chemistry and physics : MCP
Jahrgang218
Ausgabenummer16
PublikationsstatusVeröffentlicht - Aug. 2017
Peer-Review-StatusJa
Extern publiziertJa

Externe IDs

Scopus 85020089356

Schlagworte

Schlagwörter

  • BSA adsorption, cysteine, furfuryl carbamate, maleimido dextran, thin films, QUARTZ-CRYSTAL MICROBALANCE, PROTEIN ADSORPTION, CLICK CHEMISTRY, POLYMER SURFACE, NANOPARTICLES, ADHESION