Despite many years of research and rapid changes in the field of bicycle route choice modelling, a review of factors associated with cyclists’ route choice decisions is missing. This paper reviews 33 studies that employed GPS data and statistical modelling to explain the route choice behaviour of cyclists on utilitarian trips. Factors associated with bicycle route choice are divided into network, contextual, and individual factors. Findings from this review demonstrate that a refined categorisation of model attributes is essential, as the preferences vary greatly between different route attributes. Moreover, the analysis indicates a strong influence of the local context on the attribute definitions and route choice preferences. Although some commonalities between studies w.r.t. the network factors (e.g. a preference towards bicycle infrastructure or avoidance of slopes) can be determined, many results are contradictory or intertwined with other factors. Specifically, results related to intersections, land use characteristics, and interaction with other modes of transport are not always conclusive and are strongly dependent on the local context. However, the explanation of some of the unclear effects can be facilitated by incorporating contextual or individual information in the models. Based on the analysis of the existing studies, this review encourages a joint consideration of studies employing quantitative and qualitative methods to explain cyclists’ behaviour. Moreover, reporting model results in an accessible form (e.g. through relatable values or visualisation) is essential to build bridges between bicycle research and policymaking. Future research is encouraged to study cyclists’ route choice decisions from the perspective of objective well-being, quantifying and modelling the influence of factors such as safety, health, energy expenditure, or noise exposure.