Quantified CSF antibody reactivity against myelin in multiple sclerosis

Publikation: Beitrag in FachzeitschriftForschungsartikelBeigetragenBegutachtung

Beitragende

  • Xingwen Sun - , Max Planck Institute of Experimental Medicine, Georg-August-Universität Göttingen (Gemeinsame:r Erstautor:in)
  • Mostafa Bakhti - , Max Planck Institute of Experimental Medicine, Georg-August-Universität Göttingen (Gemeinsame:r Erstautor:in)
  • Dirk Fitzner - , Max Planck Institute of Experimental Medicine, Georg-August-Universität Göttingen (Gemeinsame:r Erstautor:in)
  • Mareike Schnaars - , Max Planck Institute of Experimental Medicine, Georg-August-Universität Göttingen (Autor:in)
  • Niels Kruse - , Georg-August-Universität Göttingen (Autor:in)
  • Ünal Coskun - , Deutsches Zentrum für Diabetesforschung - Paul Langerhans Institut Dresden (Partner: HMGU), Deutsches Zentrum für Diabetesforschung (DZD e.V.), Max Planck Institute of Molecular Cell Biology and Genetics (Autor:in)
  • Christiane Kremser - , Universität Bonn (Autor:in)
  • Klaus Willecke - , Universität Bonn (Autor:in)
  • Ludwig Kappos - , Universitätsspital Basel (Autor:in)
  • Jens Kuhle - , Universitätsspital Basel (Autor:in)
  • Mikael Simons - , Max Planck Institute of Experimental Medicine, Georg-August-Universität Göttingen (Autor:in)

Abstract

Background
Synthesis of clonal IgG is a consistent feature of patients with multiple sclerosis (MS). Whether oligoclonal bands (OCBs) represent unspecific disease bystanders or active components in MS pathology is an open question. The aim of this study was to develop a method to quantify and compare the reactivity of cerebrospinal fluid (CSF) antibodies from patients with and without MS.

Methods
We collected CSF from 262 patients from two different cohorts, which included 148 patients with MS and 114 with other neurological diseases (OND). We established a highly sensitive electrochemiluminescence (ECL)-based assay to measure CSF antibody reactivity against purified myelin particles and biotin anchored liposomes. The diagnostic value of the ECL score against myelin particles was assessed with receiver operating characteristic curves.

Results
CSF from patients with MS have higher reactivity toward purified myelin particles as compared to those with OND with OCBs. Using liposomes with defined lipid compositions and myelin particles from ceramide synthase 2 (CerS2) knockout mice, we find that some of the CSF antibody reactivity is directed against cerebrosides.

Conclusion
The ECL-based assay system expands the currently available toolbox for the detection of autoantibodies in MS and related diseases.

Details

OriginalspracheEnglisch
Seiten (von - bis)1116-1123
Seitenumfang8
FachzeitschriftAnnals of Clinical and Translational Neurology
Jahrgang2
Ausgabenummer12
PublikationsstatusVeröffentlicht - Nov. 2015
Peer-Review-StatusJa

Externe IDs

ORCID /0000-0003-4375-3144/work/142255279