The ionic liquid 1-ethyl-3-methyl-imidazolium acetate (EMIMOAc) is used to develop a wet-spinning process for the manufacturing of chitosan multifilament yarns on a semi-industrial pilot scale. Commercial chitosans of different qualities, properties, and deacetylation degree ≤85% are used to prepare of spinning dopes with solids content of 6.0–8.5 wt.% using EMIMOAc as a solvent. The viscometric properties are characterized by rheological tests. The manufacturing of the pure chitosan multifilament yarns of different fineness and filament count from the prepared spinning dopes is performed on a pilot wet-spinning plant. The obtained yarn properties are evaluated by optical (scanning electron microscope, light microscope), structural (infrared spectroscopy, X-ray diffraction), and textile-physical characterization. Chitosan multifilament yarns with high performance, tenacities of ~20–26 cN tex⁻¹ and textile processability in weaving, knitting and braiding processes are manufactured in this work.