Background: Complex chronic conditions in children and adolescents very often affect the nervous system. These children are frequently dependent on a wide variety of medical aids including home respiration or respiratory devices. They are often unable to communicate verbally. Frequent hospital stays as well as the use of various outpatient support services characterize their everyday life. Despite the complex care needs there has been little research on parents’ perceptions of the quality of care for these children and adolescents. Aim: The present study aimed to validate a questionnaire to assess the quality of care of children and adolescents with complex chronic neurological diseases (KoCoN-V). Methods: A prospective multicenter study was conducted. Study participants were recruited from March 2022 to October 2022 in six pediatric neurology departments. Principal component analysis as well as reliability and item analyses were employed. Results: Data from 342 patients were collected. The questionnaire was rated as appropriate by the participants in terms of its length, comprehensibility and relevance of the questions. Within the principal component analysis, a single factor structure was revealed. High correlation (Spearman’s p = −0.678, p < 0.001.) of the sum scores of the KoCoN‑V with a global measure of quality of care confirmed convergent validity. Internal consistency of the KoCoN‑V was excellent (Cronbach’s alpha = 0.905). Conclusion: The KoCoN‑V is a validated measure to assess the quality of care for children and adolescents with complex chronic neurological disorders.