Portable droplet-based real-time monitoring of pancreatic α-amylase in postoperative patients

Publikation: Beitrag in FachzeitschriftForschungsartikelBeigetragenBegutachtung

Beitragende

Abstract

Postoperative complications after pancreatic surgery are frequent and can be life-threatening. Current clinical diagnostic strategies involve time-consuming quantification of α-amylase activity in abdominal drain fluid, which is performed on the first and third postoperative day. The lack of real-time monitoring may delay adjustment of medical treatment upon complications and worsen prognosis for patients. We report a bedside portable droplet-based millifluidic device enabling real-time sensing of drain α-amylase activity for postoperative monitoring of patients undergoing pancreatic surgery. Here, a tiny amount of drain liquid of patient samples is continuously collected and co-encapsulated with a starch reagent in nanoliter-sized droplets to track the fluorescence intensity released upon reaction with α-amylase. Comparing the α-amylase levels of 32 patients, 97 % of the results of the droplet-based millifluidic system matched the clinical data. Our method reduces the α-amylase assay duration to approximately 3 min with the limit of detection 7 nmol/s·L, enabling amylase activity monitoring at the bedside in clinical real-time. The presented droplet-based platform can be extended for analysis of different body fluids, diseases, and towards a broader range of biomarkers, including lipase, bilirubin, lactate, inflammation, or liquid biopsy markers, paving the way towards new standards in postoperative patient monitoring.

Details

OriginalspracheEnglisch
Aufsatznummer116034
FachzeitschriftBiosensors and Bioelectronics
Jahrgang251
PublikationsstatusVeröffentlicht - 1 Mai 2024
Peer-Review-StatusJa

Externe IDs

PubMed 38359666

Schlagworte

Schlagwörter

  • Droplet-based microfluidics, Pancreatic surgery, Pancreatic α-amylase, Point-of-care diagnostics, Post-surgery diagnostics, Postoperative pancreatic fistula, Amylases/analysis, Humans, Pancreaticoduodenectomy/adverse effects, Pancreatic Fistula/diagnosis, alpha-Amylases, Biosensing Techniques, Pancreatic alpha-Amylases