Pleiotropic Devitalization of Renal Cancer Cells by Non-Invasive Physical Plasma: Characterization of Molecular and Cellular Efficacy

Publikation: Beitrag in FachzeitschriftForschungsartikelBeigetragenBegutachtung

Beitragende

  • Andreas Nitsch - , Universitätsklinikum Greifswald (Autor:in)
  • Caroline Sander - , Universitätsklinikum Bonn (Autor:in)
  • Benedikt Eggers - , Universitätsklinikum Bonn (Autor:in)
  • Martin Weiss - , Eberhard Karls Universität Tübingen (Autor:in)
  • Eva Egger - , Universitätsklinikum Bonn (Autor:in)
  • Franz-Josef Kramer - , Universitätsklinikum Bonn (Autor:in)
  • Holger H. H. Erb - , Klinik und Poliklinik für Urologie, Deutsche Gesellschaft für Urologie e.V. (Autor:in)
  • Alexander Mustea - , Universitätsklinikum Bonn (Autor:in)
  • Matthias B. Stope - , Universitätsklinikum Bonn, Deutsche Gesellschaft für Urologie e.V. (Autor:in)

Abstract

Renal cell carcinoma (RCC) is the third most common urological tumor and has an extremely poor prognosis after metastasis has occurred. Therapeutic options are highly restricted, primarily due to resistance to classical chemotherapeutics. The development of new, innovative therapeutic procedures is thus of great urgency. In the present study, the influence of non-invasive physical plasma (NIPP) on malignant and non-malignant renal cells is characterized. The biological efficacy of NIPP has been demonstrated in malignant renal cell lines (786-O, Caki-1) and non-malignant primary human renal epithelial cells (HREpC). The cell responses that were experimentally examined were cell growth (cell number determination, calculation of growth rate and doubling time), cell motility (scratch assay, invasiveness assay), membrane integrity (uptake of fluorescent dye, ATP release), and induction of apoptosis (TUNEL assay, caspase-3/7 assay, comet assay). A single NIPP treatment of the malignant cells significantly inhibited cell proliferation, invasiveness, and metastasis. This treatment has been attributed to the disruption of membrane functionality and the induction of apoptotic mechanisms. Comparison of NIPP sensitivity of malignant 786-O and Caki-1 cells with non-malignant HREpC cells showed significant differences. Our results suggest that renal cancer cells are significantly more sensitive to NIPP than non-malignant renal cells. Treatment with NIPP could represent a promising innovative option for the therapy of RCC and might supplement established treatment procedures. Of high clinical relevance would be the chemo-sensitizing properties of NIPP, which could potentially allow a combination of NIPP treatment with low-dose chemotherapy.

Details

OriginalspracheEnglisch
Aufsatznummer481
Seiten (von - bis)1-18
Seitenumfang18
FachzeitschriftCancers
Jahrgang15
Ausgabenummer2
PublikationsstatusVeröffentlicht - 12 Jan. 2023
Peer-Review-StatusJa

Externe IDs

Scopus 85146577659
WOS 000914583200001
PubMed 36672432
Mendeley 3c224c99-ede4-3bf6-8bda-2e1fa56ac105

Schlagworte

Ziele für nachhaltige Entwicklung

ASJC Scopus Sachgebiete

Schlagwörter

  • Apoptosis, Cold atmospheric plasma, Membrane permeability, Non-invasive physical plasma, Renal cell carcinoma

Bibliotheksschlagworte