Phenotypic screening using mouse and human stem cell-based models of neuroinflammation and gene expression analysis to study drug responses

Publikation: Beitrag in Buch/Konferenzbericht/Sammelband/GutachtenBeitrag in Buch/Sammelband/GutachtenBeigetragenBegutachtung

Beitragende

Abstract

High-throughput phenotypic screening enables the identification of new therapeutic targets even when the molecular mechanism underlying the disease is unknown. In the case of neurodegenerative disease, there is a dire need to identify new targets that can ameliorate, halt, or reverse degeneration. Stem cell-based disease models are particularly powerful tools for phenotypic screening because they use the same cell type affected in patients. Here, we describe the expansion of mouse stem cells and human induced pluripotent stem cells as well as the differentiation of these cells into neural lineages that, when exposed to neuroinflammatory stress, can be used for compound screening followed by hit identification, validation, and target deconvolution.

Details

OriginalspracheEnglisch
TitelMethods in Molecular Biology
Herausgeber (Verlag)Humana Press
Seiten21-43
Seitenumfang23
PublikationsstatusVeröffentlicht - 2019
Peer-Review-StatusJa

Publikationsreihe

ReiheMethods in Molecular Biology
Band1888
ISSN1064-3745

Externe IDs

PubMed 30519939
ORCID /0000-0002-7688-3124/work/142250053

Schlagworte

Ziele für nachhaltige Entwicklung

ASJC Scopus Sachgebiete

Schlagwörter

  • Disease modelling, Neurodegeneration, Neuroinflammation, Neuronal differentiation, Phenotypic screening, Pluripotent stem cells, Target deconvolution

Bibliotheksschlagworte