Phenotypic screening using mouse and human stem cell-based models of neuroinflammation and gene expression analysis to study drug responses
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Beitragende
Abstract
High-throughput phenotypic screening enables the identification of new therapeutic targets even when the molecular mechanism underlying the disease is unknown. In the case of neurodegenerative disease, there is a dire need to identify new targets that can ameliorate, halt, or reverse degeneration. Stem cell-based disease models are particularly powerful tools for phenotypic screening because they use the same cell type affected in patients. Here, we describe the expansion of mouse stem cells and human induced pluripotent stem cells as well as the differentiation of these cells into neural lineages that, when exposed to neuroinflammatory stress, can be used for compound screening followed by hit identification, validation, and target deconvolution.
Details
Originalsprache | Englisch |
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Titel | Methods in Molecular Biology |
Herausgeber (Verlag) | Humana Press |
Seiten | 21-43 |
Seitenumfang | 23 |
Publikationsstatus | Veröffentlicht - 2019 |
Peer-Review-Status | Ja |
Publikationsreihe
Reihe | Methods in Molecular Biology |
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Band | 1888 |
ISSN | 1064-3745 |
Externe IDs
PubMed | 30519939 |
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ORCID | /0000-0002-7688-3124/work/142250053 |
Schlagworte
Ziele für nachhaltige Entwicklung
ASJC Scopus Sachgebiete
Schlagwörter
- Disease modelling, Neurodegeneration, Neuroinflammation, Neuronal differentiation, Phenotypic screening, Pluripotent stem cells, Target deconvolution