Overexpression of ctr1300, a high-affinity copper transporter with deletion of the cytosolic C-terminus in Saccharomyces cerevisiae under excess copper, leads to disruption of transition metal homeostasis and transcriptional remodelling of cellular processes

Publikation: Beitrag in FachzeitschriftForschungsartikelBeigetragenBegutachtung

Beitragende

Abstract

In an approach to generating Saccharomyces cerevisiae strains with increased intracellular copper amounts for technical applications, we overexpressed the copper transporter CTR1 and a variant of CTR1 with a truncation in the C-terminus after the 300th amino acid (ctr1300). We determined the copper sensitivity of the generated strains and used inductively coupled plasma spectrometry analysis (ICPOES and ICPMS) to investigate the effects of overexpression of both constructs under excess copper on the cellular content of different elements in S. cerevisiae. In addition, we performed DNA microarray analysis to obtain the gene expression profile under the changed element contents. Overexpression of CTR1 increased the copper content in the cells to 160% and 78 genes were differentially regulated. Overexpression of the truncated ctr1300 resulted in an increased copper, iron and zinc content of>200% and 980 genes showed differential expression. We found that transition metal ion homeostasis was disrupted in ctr1300-overexpressing strains under excess copper and that this was combined with a transcriptional remodelling of cellular processes. Copyright (c) 2013 John Wiley & Sons, Ltd.

Details

OriginalspracheEnglisch
Seiten (von - bis)201-218
Seitenumfang18
FachzeitschriftYeast : a forum for yeast researchers
Jahrgang30
Ausgabenummer5
PublikationsstatusVeröffentlicht - Mai 2013
Peer-Review-StatusJa

Externe IDs

Scopus 84877909140

Schlagworte

Schlagwörter

  • Saccharomyces cerevisiae, transition metal, ICPOES, ICPMS, transcriptional profiling, GENE-EXPRESSION, SUPEROXIDE-DISMUTASE, IRON TRANSPORT, YEAST COPPER, GENOME-WIDE, PROTEIN, ZINC, BINDING, ENCODES, MAC1