Noninvasive metabolic profiling of cumulus cells, oocytes, and embryos via fluorescence lifetime imaging microscopy: a mini-review
Publikation: Beitrag in Fachzeitschrift › Übersichtsartikel (Review) › Beigetragen › Begutachtung
Beitragende
Abstract
A major challenge in ART is to select high-quality oocytes and embryos. The metabolism of oocytes and embryos has long been linked to their viability, suggesting the potential utility of metabolic measurements to aid in selection. Here, we review recent work on noninvasive metabolic imaging of cumulus cells, oocytes, and embryos. We focus our discussion on fluorescence lifetime imaging microscopy (FLIM) of the autofluorescent coenzymes NAD(P)H and flavine adenine dinucleotide (FAD+), which play central roles in many metabolic pathways. FLIM measurements provide quantitative information on NAD(P)H and FAD+ concentrations and engagement with enzymes, leading to a robust means of characterizing the metabolic state of cells. We argue that FLIM is a promising approach to aid in oocyte and embryo selection.
Details
Originalsprache | Englisch |
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Seiten (von - bis) | 799-810 |
Seitenumfang | 12 |
Fachzeitschrift | Human reproduction |
Jahrgang | 38 |
Ausgabenummer | 5 |
Publikationsstatus | Veröffentlicht - 1 Mai 2023 |
Peer-Review-Status | Ja |
Externe IDs
PubMed | 37015098 |
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Schlagworte
ASJC Scopus Sachgebiete
Schlagwörter
- assessments, embryo selection, fluorescence lifetime imaging microscopy, metabolism, noninvasive