Noninvasive metabolic profiling of cumulus cells, oocytes, and embryos via fluorescence lifetime imaging microscopy: a mini-review

Publikation: Beitrag in FachzeitschriftÜbersichtsartikel (Review)BeigetragenBegutachtung

Beitragende

Abstract

A major challenge in ART is to select high-quality oocytes and embryos. The metabolism of oocytes and embryos has long been linked to their viability, suggesting the potential utility of metabolic measurements to aid in selection. Here, we review recent work on noninvasive metabolic imaging of cumulus cells, oocytes, and embryos. We focus our discussion on fluorescence lifetime imaging microscopy (FLIM) of the autofluorescent coenzymes NAD(P)H and flavine adenine dinucleotide (FAD+), which play central roles in many metabolic pathways. FLIM measurements provide quantitative information on NAD(P)H and FAD+ concentrations and engagement with enzymes, leading to a robust means of characterizing the metabolic state of cells. We argue that FLIM is a promising approach to aid in oocyte and embryo selection.

Details

OriginalspracheEnglisch
Seiten (von - bis)799-810
Seitenumfang12
FachzeitschriftHuman reproduction
Jahrgang38
Ausgabenummer5
PublikationsstatusVeröffentlicht - 1 Mai 2023
Peer-Review-StatusJa

Externe IDs

PubMed 37015098

Schlagworte

Schlagwörter

  • assessments, embryo selection, fluorescence lifetime imaging microscopy, metabolism, noninvasive