No Evidence for Cardiomyocyte Number Expansion in Preadolescent Mice

Publikation: Beitrag in FachzeitschriftForschungsartikelBeigetragenBegutachtung

Beitragende

  • Kanar Alkass - , Karolinska Institutet, National Board of Forensic Medicine (Autor:in)
  • Joni Panula - , Karolinska Institutet (Autor:in)
  • Mattias Westman - , Karolinska Institutet (Autor:in)
  • Ting Di Wu - , Institut Curie, Centre national de la recherche scientifique (CNRS) (Autor:in)
  • Jean Luc Guerquin-Kern - , Institut Curie, Centre national de la recherche scientifique (CNRS) (Autor:in)
  • Olaf Bergmann - , Karolinska Institutet (Autor:in)

Abstract

Summary The magnitude of cardiomyocyte generation in the adult heart has been heavily debated. A recent report suggests that during mouse preadolescence, cardiomyocyte proliferation leads to a 40% increase in the number of cardiomyocytes. Such an expansion would change our understanding of heart growth and have far-reaching implications for cardiac regeneration. Here, using design-based stereology, we found that cardiomyocyte proliferation accounted for 30% of postnatal DNA synthesis; however, we were unable to detect any changes in cardiomyocyte number after postnatal day 11. 15N-thymidine and BrdU analyses provided no evidence for a proliferative peak in preadolescent mice. By contrast, cardiomyocyte multinucleation comprises 57% of postnatal DNA synthesis, followed by cardiomyocyte nuclear polyploidisation, contributing with 13% to DNA synthesis within the second and third postnatal weeks. We conclude that the majority of cardiomyocytes is set within the first postnatal week and that this event is followed by two waves of non-replicative DNA synthesis. This Matters Arising paper is in response to Naqvi et al. (2014), published in Cell. See also the associated Correspondence by Soonpaa et al. (2015), and the response by Naqvi et al. (2015), published in this issue.

Details

OriginalspracheEnglisch
Seiten (von - bis)1026-1036
Seitenumfang11
FachzeitschriftCell
Jahrgang163
Ausgabenummer4
PublikationsstatusVeröffentlicht - 5 Nov. 2015
Peer-Review-StatusJa
Extern publiziertJa

Externe IDs

PubMed 26544945
ORCID /0000-0003-1065-4107/work/149081853