The construction of subsurface dams for controlling seawater intrusion triggers the accumulation of nitrate upstream of a dam. This is raising the concerns about nitrate contamination in those regions of coastal aquifers that are supposed to be used as a fresh groundwater source behind a subsurface dam. Research on this subject has been mostly restricted to the use of a simplified sea boundary (e.g., static and no slope), ignoring sea level fluctuations driven by tides. In this study, the combined effect of tides and subsurface dams on nitrate pollution in upstream groundwater was examined through laboratory experiments and numerical simulations. The results revealed that the difference in the extent of nitrate contamination under various conditions (i.e., static, tidal, static with a dam, and tidal with a dam) was related to the temporal pollution behavior. In the early stage, nitrate contamination in upstream groundwater was essentially identical for different scenarios. Both tides and subsurface dams were found to increase nitrate contamination in upstream aquifers. The extent of nitrate contamination increased with higher tidal amplitudes, whereas the increment was more evident for a large tidal amplitude. The effects of tides and subsurface dams on nitrate contamination were also regulated by the locations and infiltration rates of the pollution source. Interestingly, under the joint action of tides and subsurface dams, the increment in the extent of nitrate pollution was greater than the sum of their individual effects. The increased pollutions caused by subsurface dams and tides were quantified as 9.47% and 37.22%, respectively, whereas the increased value caused by their joint action was measured as 51.10%. These findings suggest that tidal activity should not be overlooked when assessing nitrate contamination in upstream groundwater.