Migration of human melanoma cells depends on extracellular pH and Na+/H+ exchange

Publikation: Beitrag in FachzeitschriftForschungsartikelBeigetragenBegutachtung

Beitragende

  • Christian Stock - , Westfälische Wilhelms-Universität Münster (Autor:in)
  • Birgit Gassner - , Julius-Maximilians-Universität Würzburg (Autor:in)
  • Christof R. Hauck - , Julius-Maximilians-Universität Würzburg (Autor:in)
  • Hannelore Arnold - , Westfälische Wilhelms-Universität Münster (Autor:in)
  • Sabine Mally - , Westfälische Wilhelms-Universität Münster (Autor:in)
  • Johannes A. Eble - (Autor:in)
  • Peter Dieterich - , Institut für Physiologie (Autor:in)
  • Albrecht Schwab - , Westfälische Wilhelms-Universität Münster (Autor:in)

Abstract

Their glycolytic metabolism imposes an increased acid load upon tumour cells. The surplus protons are extruded by the Na+/H+ exchanger (NHE) which causes an extracellular acidification. It is not yet known by what mechanism extracellular pH (pHe) and NHE activity affect tumour cell migration and thus metastasis. We studied the impact of pHe and NHE activity on the motility of human melanoma (MV3) cells. Cells were seeded on/in collagen I matrices. Migration was monitored employing time lapse video microscopy and then quantified as the movement of the cell centre. Intracellular pH (pHi) was measured fluorometrically. Cell-matrix interactions were tested in cell adhesion assays and by the displacement of microbeads inside a collagen matrix. Migration depended on the integrin α2β1. Cells reached their maximum motility at pHe ∼7.0. They hardly migrated at pHe 6.6 or 7.5, when NHE was inhibited, or when NHE activity was stimulated by loading cells with propionic acid. These procedures also caused characteristic changes in cell morphology and pHi. The changes in pHi, however, did not account for the changes in morphology and migratory behaviour. Migration and morphology more likely correlate with the strength of cell-matrix interactions. Adhesion was the strongest at pHe 6.6. It weakened at basic pHe, upon NHE inhibition, or upon blockage of the integrin α2β1. We propose that pHe and NHE activity affect migration of human melanoma cells by modulating cell-matrix interactions. Migration is hindered when the interaction is too strong (acidic pHe) or too weak (alkaline pHe or NHE inhibition).

Details

OriginalspracheEnglisch
Seiten (von - bis)225-238
Seitenumfang14
FachzeitschriftJournal of physiology
Jahrgang567
Ausgabenummer1
PublikationsstatusVeröffentlicht - 15 Aug. 2005
Peer-Review-StatusJa

Externe IDs

PubMed 15946960
ORCID /0000-0002-3564-0193/work/175220761

Schlagworte

ASJC Scopus Sachgebiete