Micromanipulator-Assisted Subretinal Transplantation of Human Photoreceptor Reporter Cell Suspensions into Mice

Publikation: Beitrag in Buch/Konferenzbericht/Sammelband/GutachtenBeitrag in Buch/Sammelband/GutachtenBeigetragenBegutachtung

Beitragende

Abstract

Retinopathies marked by photoreceptor cell loss are the main cause of visual impairment and blindness in industrialized societies. Photoreceptor replacement strategies are examined as potential treatment options, for instance, by subretinal transplantation of photoreceptor-enriched cell suspensions, an approach which has shown beneficial effects in mouse models of retinal degeneration. As it is now possible to generate human photoreceptors through retinal organoid culture, preclinical research has shifted to human-into-mouse transplantations. Here, we describe a detailed method for human retinal organoid dissociation and fluorescent reporter-based sorting of photoreceptors. We introduce a transvitreal, micromanipulator-based transplantation technique to increase the quality and efficiency of cell injections into the mouse subretinal space, thereby increasing reproducibility and reducing experimental animal numbers. Lastly, we use a local corticosteroid to prevent immune rejection of the grafted cells in this human-into-mouse xenotransplantation setting.

Details

OriginalspracheEnglisch
TitelNeuromethods
Herausgeber (Verlag)Humana Press
Seiten81-98
Seitenumfang18
PublikationsstatusVeröffentlicht - 2023
Peer-Review-StatusJa

Publikationsreihe

ReiheNeuromethods
Band189
ISSN0893-2336

Externe IDs

ORCID /0000-0001-9467-7677/work/161888202

Schlagworte

Ziele für nachhaltige Entwicklung

Schlagwörter

  • 3R, Flow cytometry, Micromanipulator, Photoreceptor replacement, Reduction, Refinement, Reporter iPSC, Retinal organoid, Subretinal, Suspension, Transplantation