Meiotic homologue alignment and its quality surveillance are controlled by mouse HORMAD1

Publikation: Beitrag in FachzeitschriftForschungsartikelBeigetragenBegutachtung

Beitragende

Abstract

Meiotic crossover formation between homologous chromosomes (homologues) entails DNA double-strand break (DSB) formation, homology search using DSB ends, and synaptonemal-complex formation coupled with DSB repair. Meiotic progression must be prevented until DSB repair and homologue alignment are completed, to avoid the formation of aneuploid gametes. Here we show that mouse HORMAD1 ensures that sufficient numbers of processed DSBs are available for successful homology search. HORMAD1 is needed for normal synaptonemal-complex formation and for the efficient recruitment of ATR checkpoint kinase activity to unsynapsed chromatin. The latter phenomenon was proposed to be important in meiotic prophase checkpoints in both sexes. Consistent with this hypothesis, HORMAD1 is essential for the elimination of synaptonemal-complex-defective oocytes. Synaptonemal-complex formation results in HORMAD1 depletion from chromosome axes. Thus, we propose that the synaptonemal complex and HORMAD1 are key components of a negative feedback loop that coordinates meiotic progression with homologue alignment: HORMAD1 promotes homologue alignment and synaptonemal-complex formation, and synaptonemal complexes downregulate HORMAD1 function, thereby permitting progression past meiotic prophase checkpoints.

Details

OriginalspracheEnglisch
Seiten (von - bis)599-610
Seitenumfang12
FachzeitschriftNature Cell Biology
Jahrgang13
Ausgabenummer5
PublikationsstatusVeröffentlicht - Mai 2011
Peer-Review-StatusJa

Externe IDs

Scopus 79955588818
researchoutputwizard legacy.publication#51839
researchoutputwizard legacy.publication#57010
ORCID /0000-0002-4754-1707/work/162845364

Schlagworte

Schlagwörter

  • Animals\nCell Cycle Proteins/*physiology\nDNA Damage\nDNA Repair\n*Meiosis\nMice