Mechanically controlled preparation of protein intermediates in single molecule experiments

Publikation: Beitrag in FachzeitschriftForschungsartikelBeigetragenBegutachtung

Beitragende

Abstract

Single molecule mechanical experiments allow the controlled manipulation of proteins and the population of intermediate states within their high dimensional energy landscape. Here we report single molecule refolding measurements of domain 4 from the protein ddFilamin using a novel digital control scheme. First, the protein is unfolded mechanically to an intermediate state. An on-line detection scheme then detects population of the intermediate and rapidly switches to refolding conditions. This control scheme allows direct measurement of the refolding kinetics. A comparison to the results from conventional single molecule refolding schemes allows important conclusion on the structural relation between unfolding and refolding intermediates in this protein domain. (c) 2006 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Details

OriginalspracheEnglisch
Seiten (von - bis)3492-3495
Seitenumfang4
FachzeitschriftPhysica Status Solidi (A) Applied Research
Jahrgang203
Ausgabenummer14
PublikationsstatusVeröffentlicht - Nov. 2006
Peer-Review-StatusJa

Externe IDs

Scopus 34248381299
ORCID /0000-0002-6209-2364/work/142237648

Schlagworte

Schlagwörter

  • ENERGY LANDSCAPE

Bibliotheksschlagworte