Lipid domain formation and dynamics in giant unilamellar vesicles explored by fluorescence correlation spectroscopy

Publikation: Beitrag in FachzeitschriftKurze Umfrage/ÜbersichtsartikelBeigetragenBegutachtung

Beitragende

  • Nicoletta Kahya - , Technische Universität Dresden (Autor:in)
  • Dag Scherfeld - (Autor:in)
  • Kirsten Bacia - , Technische Universität Dresden (Autor:in)
  • Petra Schwille - , Professur für Biophysik (Autor:in)

Abstract

Lipids in eukaryotic cell membranes have been shown to cluster in "rafts" with different lipid/protein compositions and molecular packing. Model membranes such as giant unilamellar vesicles (GUVs) provide a key system to elucidate the physical mechanisms of raft assembly. Despite the large amount of work devoted to the detection and characterization of rafts, one of the most important pieces of information still missing in the picture of the cell membrane is dynamics: how lipids organize and move in rafts and how they modulate membrane fluidity. This missing element is of crucial importance for the trafficking at and from the periphery of the cell regulated by endo- and exocytosis and, in general, for the constant turnover which redistributes membrane components. Here, we review studies of combined confocal fluorescence microscopy and fluorescence correlation spectroscopy on lipid dynamics and organization in rafts assembled in GUVs prepared from various lipid mixtures which are relevant to the problem of raft formation.

Details

OriginalspracheEnglisch
Seiten (von - bis)77-89
Seitenumfang13
FachzeitschriftJournal of Structural Biology
Jahrgang147
Ausgabenummer1
PublikationsstatusVeröffentlicht - Juli 2004
Peer-Review-StatusJa

Externe IDs

PubMed 15109608

Schlagworte

ASJC Scopus Sachgebiete

Schlagwörter

  • Cholesterol, Confocal fluorescence microscopy, Fluorescence correlation spectroscopy, GUVs, Lipid rafts, Phosphatidylcholine, Sphingomyelin