Lineage-specific enhancers activate self-renewal genes in macrophages and embryonic stem cells
Publikation: Beitrag in Fachzeitschrift › Forschungsartikel › Beigetragen › Begutachtung
Beitragende
Abstract
Differentiated macrophages can self-renew in tissues and expand long term in culture, but the gene regulatory mechanisms that accomplish self-renewal in the differentiated state have remained unknown. Here we show that in mice, the transcription factors MafB and c-Maf repress a macrophage-specific enhancer repertoire associated with a gene network that controls self-renewal. Single-cell analysis revealed that, in vivo, proliferating resident macrophages can access this network by transient down-regulation of Maf transcription factors. The network also controls embryonic stem cell self-renewal but is associated with distinct embryonic stem cell-specific enhancers. This indicates that distinct lineage-specific enhancer platforms regulate a shared network of genes that control self-renewal potential in both stem and mature cells.
Details
Originalsprache | Englisch |
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Aufsatznummer | aad5510 |
Fachzeitschrift | Science |
Jahrgang | 351 |
Ausgabenummer | 6274 |
Publikationsstatus | Veröffentlicht - 12 Feb. 2016 |
Peer-Review-Status | Ja |
Externe IDs
PubMedCentral | PMC4811353 |
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Scopus | 84958191207 |
Schlagworte
Schlagwörter
- Animals, Cell Differentiation/genetics, Cell Lineage/genetics, Cell Proliferation, Cells, Cultured, Down-Regulation, Embryonic Stem Cells/cytology, Enhancer Elements, Genetic/physiology, Gene Expression Regulation, Gene Regulatory Networks, Macrophages/cytology, MafB Transcription Factor/metabolism, Mice, Proto-Oncogene Proteins c-maf/metabolism, Single-Cell Analysis, Transcriptional Activation