Lineage specification of hematopoietic stem cells: mathematical modeling and biological implications
Publikation: Beitrag in Fachzeitschrift › Forschungsartikel › Beigetragen › Begutachtung
Beitragende
Abstract
Lineage specification of hematopoietic stem cells is considered a progressive restriction in lineage potential. This view is consistent with observations that differentiation and lineage specification is preceded by a low-level coexpression of lineage specific, potentially antagonistic genes in early progenitor cells. This coexistence, commonly referred to as priming, disappears in the course of differentiation when certain lineage-restricted genes are upregulated while others are downregulated. Based on this phenomenological description, we propose a quantitative model that describes lineage specification as a competition process between different interacting lineage propensities. The competition is governed by environmental stimuli promoting a drift from a multipotent coexpression to the dominance of one lineage. The assumption of a context-dependent intracellular differentiation control is consistently embedded into our previously proposed model of hematopoietic stem cell organization. The extended model, which comprises self-renewal and lineage specification, is verified using available data on the lineage specification potential of primary hematopoietic stem cells and on the differentiation kinetics of the FDCP-mix cell line. The model provides a number of experimentally testable predictions. From our results, we conclude that lineage specification is best described as a flexible and temporally extended process in which lineage commitment emerges as the result of a sequence of small decision steps. The proposed model provides a novel systems biological view on the functioning of lineage specification in adult tissue stem cells and its connections to the self-renewal properties of these cells. Disclosure of potential conflicts of interest is found at the end of this article.
Details
Originalsprache | Englisch |
---|---|
Seiten (von - bis) | 1791-9 |
Seitenumfang | 9 |
Fachzeitschrift | Stem cells (Dayton, Ohio) |
Jahrgang | 25 |
Ausgabenummer | 7 |
Publikationsstatus | Veröffentlicht - Juli 2007 |
Peer-Review-Status | Ja |
Extern publiziert | Ja |
Externe IDs
Scopus | 34547232294 |
---|---|
ORCID | /0000-0002-2524-1199/work/142251521 |
Schlagworte
Schlagwörter
- Animals, Cell Differentiation, Cell Lineage, Cells, Cultured, Computer Simulation, Hematopoietic Stem Cells/cytology, Humans, Mice, Models, Biological