Twenty-five popular landscape trees in Shanghai were selected and seven indicators of victimization, chlorophyll, electrical conductivity, specific leaf mass, net photosynthetic rate, malondialdehyde and proline content were measured in the flood simulation experiments. Then, the resistance spectrums of the 25 landscape trees were scheduled. Finally, a method for predicting the resistance of landscape trees via the level of photosynthesis was put forward, giving priority to chlorophyll, specific leaf mass and net photosynthetic rate indicators. According to the level of waterlogging resistance, 25 tree species were divided into four types: strong waterlogging resistance, relatively waterlogging resistance, general waterlogging resistance and weak-waterlogging resistance.