Ketoprofen-Based Polymer-Drug Nanoparticles Provide Anti-Inflammatory Properties to HA/Collagen Hydrogels

Publikation: Beitrag in FachzeitschriftForschungsartikelBeigetragenBegutachtung

Beitragende

  • Norbert Halfter - , Professur für Biomaterialien (Autor:in)
  • Eva Espinosa-Cano - , CSIC - Instituto de Ciencia y Tecnología de Polímeros (ICTP), Instituto de Salud Carlos III (Autor:in)
  • Gloria María Pontes-Quero - , CSIC - Instituto de Ciencia y Tecnología de Polímeros (ICTP), Instituto de Salud Carlos III (Autor:in)
  • Rosa Ana Ramírez-Jiménez - , CSIC - Instituto de Ciencia y Tecnología de Polímeros (ICTP), Instituto de Salud Carlos III (Autor:in)
  • Christiane Heinemann - , Professur für Biomaterialien (Autor:in)
  • Stephanie Möller - , INNOVENT e. V. Technology Development (Autor:in)
  • Matthias Schnabelrauch - , INNOVENT e. V. Technology Development (Autor:in)
  • Hans-Peter Wiesmann - , Professur für Biomaterialien (Autor:in)
  • Vera Hintze - , Professur für Biomaterialien (Autor:in)
  • Maria Rosa Aguilar - , CSIC - Instituto de Ciencia y Tecnología de Polímeros (ICTP), Instituto de Salud Carlos III (Autor:in)

Abstract

Current limitations of wound dressings for treating chronic wounds require the development of novel approaches. One of these is the immune-centered approach, which aims to restore the pro-regenerative and anti-inflammatory properties of macrophages. Under inflammatory conditions, ketoprofen nanoparticles (KT NPs) can reduce pro-inflammatory markers of macrophages and increase anti-inflammatory cytokines. To assess their suitability as part of wound dressings, these NPs were combined with hyaluronan (HA)/collagen-based hydro- (HGs) and cryogels (CGs). Different HA and NP concentrations and loading techniques for NP incorporation were used. The NP release, gel morphology, and mechanical properties were studied. Generally, colonialization of the gels with macrophages resulted in high cell viability and proliferation. Furthermore, direct contact of the NPs to the cells reduced the level of nitric oxide (NO). The formation of multinucleated cells on the gels was low and further decreased by the NPs. For the HGs that produced the highest reduction in NO, extended ELISA studies showed reduced levels of the pro-inflammatory markers PGE2, IL-12 p40, TNF-α, and IL-6. Thus, HA/collagen-based gels containing KT NPs may represent a novel therapeutic approach for treating chronic wounds. Whether effects observed in vitro translate into a favorable profile on skin regeneration in vivo will require rigorous testing.

Details

OriginalspracheEnglisch
Aufsatznummer160
Seitenumfang21
FachzeitschriftJournal of Functional Biomaterials
Jahrgang14
Ausgabenummer3
PublikationsstatusVeröffentlicht - 17 März 2023
Peer-Review-StatusJa

Externe IDs

Scopus 85151153893
ORCID /0000-0002-5611-9903/work/170586996

Schlagworte