Isolation of polymorphic microsatellite loci in the clear lake gnat and two other phantom midge species of the genus Chaoborus (Diptera: Chaoboridae)

Publikation: Beitrag in FachzeitschriftForschungsartikelBeigetragenBegutachtung

Beitragende

  • T. U. Berendonk - , Fakultät Biologie, Max Planck Institute for Evolutionary Biology, Imperial College London, Universität Leipzig (Autor:in)
  • K. Dobson - , Imperial College London (Autor:in)

Abstract

Chaoborus is of great interest to many freshwater ecologists. The adults can become pests in certain areas in North America and the larvae are an important food source for fish. In this preliminary study, we identified variable microsatellite loci in three species: Chaoborus astictopus (H E = 0.52-0.76), Chaoborus americanus (HE = 0.46-0.80) and Chaoborus punctipennis (HE = 0.66-0.81). Using a biotin/streptavidin capture technique of repetitive sequences in a 96-well format, we obtained microsatellite-enriched genomic libraries for all three species and identified six polymorphic microsatellite markers for each species. None of the primers did yield a polymerase chain reaction fragment in a cross-species test.

Details

OriginalspracheEnglisch
Seiten (von - bis)17-19
Seitenumfang3
FachzeitschriftMolecular Ecology Notes
Jahrgang6
Ausgabenummer1
PublikationsstatusVeröffentlicht - März 2006
Peer-Review-StatusJa

Externe IDs

ORCID /0000-0002-9301-1803/work/161409792

Schlagworte

Schlagwörter

  • C. americanus, C. astictopus, C. punctipennis, Chaoborus, Freshwater, Microsatellites