Effects of various geometrical and physical factors, as well as the method of data reduction (analysis of experimental force–displacement curves) on the values of local interfacial strength parameters (local IFSS, τ_d, and critical energy release rate, G_ic) determined by means of a single fiber pull-out test are discussed. Experimental results of our pull-out tests on several fiber–polymer matrix systems showed that τ_d and G_ic weakly depended on geometrical factors. However, the pull-out test appeared to be sensitive to the conditions of specimen formation and testing, such as changing the nature of the contacting surfaces (fiber sizing) and the fiber pull-out rate. Of several methods of τ_d and G_ic determination from a force–displacement curve, the most reliable and reproducible one is the approach based on the values of the maximum force recorded in a pull-out test and the interfacial frictional force immediately after fiber debonding.