Increased survivin transcript levels: An independent negative predictor of survival in soft tissue sarcoma patients
Publikation: Beitrag in Fachzeitschrift › Forschungsartikel › Beigetragen › Begutachtung
Beitragende
Abstract
Survivin, a recently identified inhibitor of apoptosis protein (IAP), is expressed in diverse embryonic tissues and in various human cancers. We have investigated the quantitative expression of survivin mRNA by a sensitive TaqMan™-based RT-PCR assay in tissue samples from 94 patients with soft tissue sarcomas (STS). Survivin transcript levels were measured and normalized to GAPDH transcripts. By using a multivariate Cox regression analysis, we found an inverse correlation between the level of survivin mRNA (ratio >2 zmol survivin/amol GAPDH) and the rate of overall survival (p = 0.009, RR = 2.7). Survivin transcript variants as detected by qualitative RT-PCR analysis were revealed in 36 of 56 STS patients (64%). Only survivin ΔEx3 and/or full-length survivin variants but not survivin 2B were identified. Our results suggest that a higher level of survivin mRNA is an independent predictor of survival for STS patients.
Details
Originalsprache | Englisch |
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Seiten (von - bis) | 360-363 |
Seitenumfang | 4 |
Fachzeitschrift | International journal of cancer |
Jahrgang | 95 |
Ausgabenummer | 6 |
Publikationsstatus | Veröffentlicht - 20 Nov. 2001 |
Peer-Review-Status | Ja |
Externe IDs
PubMed | 11668517 |
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Schlagworte
Ziele für nachhaltige Entwicklung
ASJC Scopus Sachgebiete
Schlagwörter
- Multivariate analysis, Quantitative RT-PCR, Soft tissue sarcoma, Survivin, TaqMan™