Improving Blind Spot Denoising for Microscopy

Publikation: Beitrag in Buch/Konferenzbericht/Sammelband/GutachtenBeitrag in KonferenzbandBeigetragenBegutachtung

Beitragende

  • Anna S. Goncharova - , Max Planck Institute of Molecular Cell Biology and Genetics, Zentrum für Systembiologie Dresden (CSBD) (Autor:in)
  • Alf Honigmann - , Max Planck Institute of Molecular Cell Biology and Genetics (Autor:in)
  • Florian Jug - , Max Planck Institute of Molecular Cell Biology and Genetics, Zentrum für Systembiologie Dresden (CSBD), Human Technopole (Autor:in)
  • Alexander Krull - , Max Planck Institute of Molecular Cell Biology and Genetics, Zentrum für Systembiologie Dresden (CSBD), Max-Planck-Institut für Physik komplexer Systeme (Autor:in)

Abstract

Many microscopy applications are limited by the total amount of usable light and are consequently challenged by the resulting levels of noise in the acquired images. This problem is often addressed via (supervised) deep learning based denoising. Recently, by making assumptions about the noise statistics, self-supervised methods have emerged. Such methods are trained directly on the images that are to be denoised and do not require additional paired training data. While achieving remarkable results, self-supervised methods can produce high-frequency artifacts and achieve inferior results compared to supervised approaches. Here we present a novel way to improve the quality of self-supervised denoising. Considering that light microscopy images are usually diffraction-limited, we propose to include this knowledge in the denoising process. We assume the clean image to be the result of a convolution with a point spread function (PSF) and explicitly include this operation at the end of our neural network. As a consequence, we are able to eliminate high-frequency artifacts and achieve self-supervised results that are very close to the ones achieved with traditional supervised methods.

Details

OriginalspracheEnglisch
TitelComputer Vision – ECCV 2020 Workshops, Proceedings
Redakteure/-innenAdrien Bartoli, Andrea Fusiello
Herausgeber (Verlag)Springer Science and Business Media B.V.
Seiten380-393
Seitenumfang14
ISBN (Print)9783030664145
PublikationsstatusVeröffentlicht - 2020
Peer-Review-StatusJa
Extern publiziertJa

Publikationsreihe

ReiheLecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics)
Band12535 LNCS
ISSN0302-9743

Konferenz

TitelWorkshops held at the 16th European Conference on Computer Vision, ECCV 2020
Dauer23 - 28 August 2020
StadtGlasgow
LandGroßbritannien/Vereinigtes Königreich

Externe IDs

ORCID /0000-0003-0475-3790/work/161889537

Schlagworte

Schlagwörter

  • CNN, Deconvolution, Denoising, Light microscopy