Honeycomb cores made of phenol resin impregnated paper are widely used in sandwich structures of aircraft. Owing to the rather weak core material, this kind of sandwich is prone to a range of damages as a result of impact loading which may accidentally occur during operation. When employed in aircraft these structures have to be damage tolerant. This means barely visible damages have to be considered in the structural design process. In the standard design process for damage tolerant aircraft structures especially the effect of impacts has to be considered but is mostly investigated on unloaded sandwich components. However, aircraft structures are not completely stress free, even when the aircraft is in parking position. At least the structural weight will cause a permanent static preload. In the literature there are only few investigations dealing with the impact behaviour of preloaded honeycomb sandwich structures. In general, studies on composite structures have shown that tension preloads lead to higher structural impact resistance, while prestressing in compression reduces mechanical properties such as impact strength and stiffness of the structure. For this reason, a compression preload is considered as the more critical load case. In order to evaluate the effect of static compression preloads an experimental study on five different honeycomb sandwich configurations was conducted. The experimental investigations showed that even low preloads cause a higher structural damage area, which results in a lower residual strength of the structure. The results indicated that it is recommendable to consider structural compression preloads in the aircraft structural design process.