Identification of nonvisual photomotor response cells in the vertebrate hindbrain

Publikation: Beitrag in FachzeitschriftForschungsartikelBeigetragenBegutachtung

Beitragende

  • David Kokel - , Harvard Medical School (HMS) (Autor:in)
  • Timothy W Dunn - , Harvard University (Autor:in)
  • Misha B Ahrens - , Harvard University (Autor:in)
  • Rüdiger Alshut - (Autor:in)
  • Chung Yan J Cheung - (Autor:in)
  • Louis Saint-Amant - (Autor:in)
  • Giancarlo Bruni - (Autor:in)
  • Rita Mateus - , Harvard Medical School (HMS) (Autor:in)
  • Tjakko J van Ham - (Autor:in)
  • Tomoya Shiraki - (Autor:in)
  • Yoshitaka Fukada - (Autor:in)
  • Daisuke Kojima - (Autor:in)
  • Jing-Ruey J Yeh - (Autor:in)
  • Ralf Mikut - (Autor:in)
  • Johannes von Lintig - (Autor:in)
  • Florian Engert - (Autor:in)
  • Randall T Peterson - (Autor:in)

Abstract

Nonvisual photosensation enables animals to sense light without sight. However, the cellular and molecular mechanisms of nonvisual photobehaviors are poorly understood, especially in vertebrate animals. Here, we describe the photomotor response (PMR), a robust and reproducible series of motor behaviors in zebrafish that is elicited by visual wavelengths of light but does not require the eyes, pineal gland, or other canonical deep-brain photoreceptive organs. Unlike the relatively slow effects of canonical nonvisual pathways, motor circuits are strongly and quickly (seconds) recruited during the PMR behavior. We find that the hindbrain is both necessary and sufficient to drive these behaviors. Using in vivo calcium imaging, we identify a discrete set of neurons within the hindbrain whose responses to light mirror the PMR behavior. Pharmacological inhibition of the visual cycle blocks PMR behaviors, suggesting that opsin-based photoreceptors control this behavior. These data represent the first known light-sensing circuit in the vertebrate hindbrain.

Details

OriginalspracheEnglisch
Seiten (von - bis)3834-43
Seitenumfang10
FachzeitschriftThe Journal of neuroscience : the official journal of the Society for Neuroscience
Jahrgang33
Ausgabenummer9
PublikationsstatusVeröffentlicht - 27 Feb. 2013
Peer-Review-StatusJa
Extern publiziertJa

Externe IDs

PubMedCentral PMC3600642
ORCID /0000-0002-6023-3880/work/153110497
Scopus 84874616928

Schlagworte

Schlagwörter

  • Age Factors, Analysis of Variance, Animals, Biomechanical Phenomena, Biophysics, Calcium/metabolism, Embryo, Nonmammalian, Female, Male, Microscopy, Confocal, Morpholinos/pharmacology, Movement/drug effects, Muscle Cells/drug effects, Neural Pathways/drug effects, Opsins/chemistry, Photic Stimulation, Photoreceptor Cells, Vertebrate/drug effects, Rhombencephalon/cytology, Stereotyped Behavior/drug effects, Time Factors, Zebrafish