Human sulfite oxidase electrochemistry on gold nanoparticles modified electrode

Publikation: Beitrag in FachzeitschriftForschungsartikelBeigetragenBegutachtung

Beitragende

  • Stefano Frasca - , Universität Potsdam (Autor:in)
  • Oscar Rojas - , Universität Potsdam (Autor:in)
  • Johannes Salewski - , Technische Universität Berlin (Autor:in)
  • Bettina Neumann - , Universität Potsdam (Autor:in)
  • Konstanze Stiba - , Universität Potsdam (Autor:in)
  • Inez M. Weidinger - , Technische Universität Berlin (Autor:in)
  • Brigitte Tiersch - , Universität Potsdam (Autor:in)
  • Silke Leimkühler - , Universität Potsdam (Autor:in)
  • Joachim Koetz - , Universität Potsdam (Autor:in)
  • Ulla Wollenberger - , Universität Potsdam (Autor:in)

Abstract

The present study reports a facile approach for sulfite biosensing, based on enhanced direct electron transfer of a human sulfite oxidase (hSO) immobilized on a gold nanoparticles modified electrode. The spherical core shell AuNPs were prepared via a new method by reduction of HAuCl4 with branched poly(ethyleneimine) in an ionic liquids resulting particles with a diameter less than 10nm. These nanoparticles were covalently attached to a mercaptoundecanoic acid modified Au-electrode where then hSO was adsorbed and an enhanced interfacial electron transfer and electrocatalysis was achieved. UV/Vis and resonance Raman spectroscopy, in combination with direct protein voltammetry, are employed for the characterization of the system and reveal no perturbation of the structural integrity of the redox protein. The proposed biosensor exhibited a quick steady-state current response, within 2s, a linear detection range between 0.5 and 5.4μM with a high sensitivity (1.85nAμM-1). The investigated system provides remarkable advantages in the possibility to work at low applied potential and at very high ionic strength. Therefore these properties could make the proposed system useful in the development of bioelectronic devices and its application in real samples.

Details

OriginalspracheEnglisch
Seiten (von - bis)33-41
Seitenumfang9
FachzeitschriftBioelectrochemistry
Jahrgang87
PublikationsstatusVeröffentlicht - Okt. 2012
Peer-Review-StatusJa
Extern publiziertJa

Externe IDs

PubMed 22209453

Schlagworte

Schlagwörter

  • Direct electron transfer, Gold nanoparticle, Human sulfite oxidase, Ionic liquid, Sulfite biosensor