In this study, stainless steel substrates were treated with laser interference patterns using burst of pulses. The main objective is to evaluate their influence in the ablation mechanism using the Direct Laser Interference Patterning method. Using a 10 ps infrared laser system operating with low and high fluence levels and different pulse overlaps, a lower efficiency in the ablation process was observed for the low energy case, while at high fluences an improvement of the ablation efficiency up to 31% was observed. In addition, it was found that overlaps higher than 97.6% significantly deteriorate the pattern quality.