Histopathological analysis of cellular localization of cathepsins in abdominal aortic aneurysm wall

Publikation: Beitrag in FachzeitschriftForschungsartikelBeigetragenBegutachtung

Beitragende

  • Fabian Lohoefer - , Technische Universität München (Autor:in)
  • Christian Reeps - , Klinik und Poliklinik für Viszeral- Thorax- und Gefäßchirurgie, Klinikum der Ludwig-Maximilians-Universität (LMU) München, Technische Universität München (Autor:in)
  • Christina Lipp - , Technische Universität München (Autor:in)
  • Martina Rudelius - , Technische Universität München (Autor:in)
  • Alexander Zimmermann - , Technische Universität München (Autor:in)
  • Stefan Ockert - , Technische Universität München (Autor:in)
  • Hans Henning Eckstein - , Technische Universität München (Autor:in)
  • Jaroslav Pelisek - , Technische Universität München (Autor:in)

Abstract

An important feature of abdominal aortic aneurysm (AAA) is the destruction of vessel wall, especially elastin and collagen. Besides matrix metalloproteinases, cathepsins are the most potent elastolytic enzymes. The expression of cathepsins with known elastolytic and collagenolytic activities in the individual cells within AAA has not yet been determined. The vessel wall of 32 AAA patients and 10 organ donors was analysed by immunohistochemistry for expression of cathepsins B, D, K, L and S, and cystatin C in all cells localized within AAA. Luminal endothelial cells (ECs) of AAA were positive for cathepsin D and partially for cathepsins B, K and S. Endothelial cells of the neovessels and smooth muscle cells in the media were positive for all cathepsins tested, especially for cathepsin B. In the inflammatory infiltrate all cathepsins were expressed in the following pattern: B > D = S > K = L. Macrophages showed the highest staining intensity for all cathepsins. Furthermore, weak overall expression of cystatin C was observed in all the cells localized in the AAA with the exception of the ECs. There is markedly increased expression of the various cathepsins within the AAA wall compared to healthy aorta. Our data are broadly consistent with a role for cathepsins in AAA; and demonstrate expression of cathepsins D, B and S in phagocytic cells in the inflammatory infiltrate; and also may reveal a role for cathepsin B in lymphocytes.

Details

OriginalspracheEnglisch
Seiten (von - bis)252-258
Seitenumfang7
FachzeitschriftInternational Journal of Experimental Pathology
Jahrgang93
Ausgabenummer4
PublikationsstatusVeröffentlicht - Aug. 2012
Peer-Review-StatusJa

Externe IDs

PubMed 22804761

Schlagworte

Schlagwörter

  • Abdominal aortic aneurysm, Cathepsins, Cystatin C, Proteolytic degradation