Hedgehog morphogen gradient is robust towards variations in tissue morphology in Drosophila
Publikation: Beitrag in Fachzeitschrift › Forschungsartikel › Beigetragen › Begutachtung
Beitragende
Abstract
During tissue development, gradients of secreted signaling molecules known as morphogens provide cells with positional information. The mechanisms underlying morphogen spreading have been widely studied, however, it remains largely unexplored whether the shape of morphogen gradients is influenced by tissue morphology. Here, we developed an analysis pipeline to quantify the distribution of proteins within a curved tissue. We applied it to the Hedgehog morphogen gradient in the Drosophila wing and eye-antennal imaginal discs, which are flat and curved tissues, respectively. Despite a different expression profile, the slope of the Hedgehog gradient was comparable between the two tissues. Moreover, inducing ectopic folds in wing imaginal discs did not affect the slope of the Hedgehog gradient. Suppressing curvature in the eye-antennal imaginal disc also did not alter the Hedgehog gradient slope but led to ectopic Hedgehog expression. In conclusion, through the development of an analysis pipeline that allows quantifying protein distribution in curved tissues, we show that the Hedgehog gradient is robust towards variations in tissue morphology.
Details
Originalsprache | Englisch |
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Aufsatznummer | 8454 |
Seitenumfang | 13 |
Fachzeitschrift | Scientific reports |
Jahrgang | 13 |
Ausgabenummer | 1 |
Publikationsstatus | Veröffentlicht - 25 Mai 2023 |
Peer-Review-Status | Ja |
Externe IDs
PubMed | 37231029 |
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Schlagworte
Forschungsprofillinien der TU Dresden
DFG-Fachsystematik nach Fachkollegium
Fächergruppen, Lehr- und Forschungsbereiche, Fachgebiete nach Destatis
ASJC Scopus Sachgebiete
Schlagwörter
- Drosophila, Cell biology, Developmental biology