Hedgehog morphogen gradient is robust towards variations in tissue morphology in Drosophila

Publikation: Beitrag in FachzeitschriftForschungsartikelBeigetragenBegutachtung

Beitragende

Abstract

During tissue development, gradients of secreted signaling molecules known as morphogens provide cells with positional information. The mechanisms underlying morphogen spreading have been widely studied, however, it remains largely unexplored whether the shape of morphogen gradients is influenced by tissue morphology. Here, we developed an analysis pipeline to quantify the distribution of proteins within a curved tissue. We applied it to the Hedgehog morphogen gradient in the Drosophila wing and eye-antennal imaginal discs, which are flat and curved tissues, respectively. Despite a different expression profile, the slope of the Hedgehog gradient was comparable between the two tissues. Moreover, inducing ectopic folds in wing imaginal discs did not affect the slope of the Hedgehog gradient. Suppressing curvature in the eye-antennal imaginal disc also did not alter the Hedgehog gradient slope but led to ectopic Hedgehog expression. In conclusion, through the development of an analysis pipeline that allows quantifying protein distribution in curved tissues, we show that the Hedgehog gradient is robust towards variations in tissue morphology.

Details

OriginalspracheEnglisch
Aufsatznummer8454
Seitenumfang13
FachzeitschriftScientific reports
Jahrgang13
Ausgabenummer1
PublikationsstatusVeröffentlicht - 25 Mai 2023
Peer-Review-StatusJa

Externe IDs

PubMed 37231029

Schlagworte

DFG-Fachsystematik nach Fachkollegium

Fächergruppen, Lehr- und Forschungsbereiche, Fachgebiete nach Destatis

ASJC Scopus Sachgebiete

Schlagwörter

  • Drosophila, Cell biology, Developmental biology

Bibliotheksschlagworte