Glyphosate based herbicides are the most commonly used herbicide in the world. We aimed to determine whether glyphosate (Gly) induces epithelial mesenchymal transition (EMT) - related changes in a human endometrial carcinoma cell line (Ishikawa cells), and whether the estrogen receptor (ER) pathway is involved in these changes. Ishikawa cells were exposed to Gly (0.2 μM and 2 μM) or 17β-estradiol (E2: 10-9 M). We detected that Gly increased cell migration and invasion ability compared to vehicle, as did E2. Moreover, a down regulation of E-cadherin mRNA expression was determined in response to Gly, similar to E2-effects. These results show that Gly promotes EMT-related changes in Ishikawa cells. When an ER antagonist (Fulvestrant: 10-7 M) was co-administrated with Gly, all changes were reversed, suggesting that Gly might promote EMT-related changes via ER-dependent pathway. Our results are interesting evidences of Gly effects on endometrial cancer progression via the ER-dependent pathway.
|Fachzeitschrift||Molecular and cellular endocrinology|
|Publikationsstatus||Veröffentlicht - 15 Juni 2020|
Ziele für nachhaltige Entwicklung
- Cadherins/genetics, Cell Line, Tumor, Cell Movement/drug effects, Cell Survival/drug effects, Down-Regulation/drug effects, Endometrium/drug effects, Epithelial-Mesenchymal Transition/drug effects, Female, Fulvestrant/pharmacology, Glycine/analogs & derivatives, Humans, Neoplasm Invasiveness, RNA, Messenger/genetics, Receptors, Estrogen/metabolism, Signal Transduction/drug effects