Glycodendrimer PPI as a Potential Drug in Chronic Lymphocytic Leukaemia. The Influence of Glycodendrimer on Apoptosis in <i>In Vitro</i> B-CLL Cells Defined by Microarrays

Publikation: Beitrag in FachzeitschriftForschungsartikelBeigetragenBegutachtung

Beitragende

  • Ida Franiak-Pietryga - , Medical University of Łódź (Autor:in)
  • Ewelina Ziolkowska - , Medical University of Łódź (Autor:in)
  • Barbara Ziemba - , Lodz University of Technology (Autor:in)
  • Dietmar Appelhans - , Leibniz-Institut für Polymerforschung Dresden (Autor:in)
  • Henryk Maciejewski - , Wrocław University of Science and Technology (Autor:in)
  • Brigitte Voit - , Professur für Organische Chemie der Polymere (gB/IPF) (MTC3), Leibniz-Institut für Polymerforschung Dresden (Autor:in)
  • Aleksandra Kaczmarek - , Lab Clin & Transplant Immunol & Genet (Autor:in)
  • Tadeusz Roba - , Medical University of Łódź (Autor:in)
  • Barbara Klajnert-Maculewicz - , Lodz University of Technology (Autor:in)
  • Barbara Cebula-Obrzut - , Medical University of Łódź (Autor:in)
  • Piotr Smolewski - , Medical University of Łódź (Autor:in)
  • Maciej Borowiec - , Medical University of Łódź (Autor:in)
  • Maria Bryszewska - , Lodz University of Technology (Autor:in)

Abstract

Background: Chronic lymphocytic leukaemia (CLL) cells are characterized by failures in the apoptosis pathway and increased proliferation, resulting in the progressive accumulation of B-lymphocytes in blood. Despite the wide range of antileukaemic drugs, CLL remains an incurable disease. However, a breakthrough is expected which will allow more effective treatment.Objective: The study investigates the influence of poly(propyleneimine) (PPI) dendrimer with peripheral amino groups, 30% of which were coated with maltotriose (PPI-G4-OS-Mal-III), on CLL cells, and demonstrates that it acts through the induction of the apoptotic mechanism. It is important to note that the dendrimer was used as a drug itself and not as a drug carrier.Method: CLL and normal lymphocytes were treated in vitro with the dendrimer, either alone or in combination with fludarabine (FA). The percentages of apoptotic and necrotic cells, and the protein expression, were checked using a flow cytometer. Gene expression was screened using a two-colour microarray with 60-mer probes.Results: The results confirm that PPI-G4-OS-Mal-III influences the viability of CLL cells in vitro and does not exert any significant harmful effect on normal lymphocytes. The dendrimer appears to significantly influence gene and protein expression in CLL cells.Conclusion: Since dendrimers can be specifically targeted, they may be very effective in CLL therapy, especially since in vitro PPI-G4-OS-Mal-III has been found to have stronger effect than fludarabine..

Details

OriginalspracheDeutsch
Seiten (von - bis)102-114
Seitenumfang13
FachzeitschriftAnti-cancer agents in medicinal chemistry
Jahrgang17
Ausgabenummer1
PublikationsstatusVeröffentlicht - 2017
Peer-Review-StatusJa

Externe IDs

PubMed 27349446
Scopus 85021850442
ORCID /0000-0002-4531-691X/work/148608074

Schlagworte

Schlagwörter

  • Apoptosis, chronic lymphocytic leukaemia (CLL), Dendrimers, Gene expression, Glycodendrimers, poly(propylene imine) (PPI), Protein expression, Toxicity