Genome-wide RNAi screen identifies genes involved in intestinal pathogenic bacterial infection

Publikation: Beitrag in FachzeitschriftForschungsartikelBeigetragenBegutachtung

Beitragende

  • Shane J.F. Cronin - (Autor:in)
  • Nadine T. Nehme - (Autor:in)
  • Stefanie Limmer - , Professur für Zoologie und Tierphysiologie (Autor:in)
  • Samuel Liegeois - (Autor:in)
  • J. Andrew Pospisilik - (Autor:in)
  • Daniel Schramek - (Autor:in)
  • Andreas Leibbrandt - (Autor:in)
  • Ricardo Matos De Simoes - (Autor:in)
  • Susanne Gruber - (Autor:in)
  • Urszula Puc - (Autor:in)
  • Ingo Ebersberger - (Autor:in)
  • Tamara Zoranovic - (Autor:in)
  • G. Gregory Neely - (Autor:in)
  • Arndt Von Haeseler - (Autor:in)
  • Dominique Ferrandon - (Autor:in)
  • Josef M. Penninger - (Autor:in)

Abstract

Innate immunity represents the first line of defense in animals. We report a genome-wide in vivo Drosophila RNA interference screen to uncover genes involved in susceptibility or resistance to intestinal infection with the bacterium Serratia marcescens. We first employed whole-organism gene suppression, followed by tissue-specific silencing in gut epithelium or hemocytes to identify several hundred genes involved in intestinal antibacterial immunity. Among the pathways identified, we showed that the JAK-STAT signaling pathway controls host defense in the gut by regulating stem cell proliferation and thus epithelial cell homeostasis. Therefore, we revealed multiple genes involved in antibacterial defense and the regulation of innate immunity.

Details

OriginalspracheEnglisch
Seiten (von - bis)340-343
Seitenumfang4
FachzeitschriftScience
Jahrgang325
Ausgabenummer5938
PublikationsstatusVeröffentlicht - 17 Juli 2009
Peer-Review-StatusJa

Externe IDs

PubMed 19520911
Scopus 67650831470

Schlagworte