Genome and transcriptome profiles of CD133-positive colorectal cancer cells

Publikation: Beitrag in FachzeitschriftForschungsartikelBeigetragenBegutachtung

Beitragende

  • Timo Gaiser - , National Cancer Institute (NCI) (Autor:in)
  • Jordi Camps - , National Cancer Institute (NCI) (Autor:in)
  • Sandra Meinhardt - , National Cancer Institute (NCI) (Autor:in)
  • Danny Wangsa - , National Cancer Institute (NCI) (Autor:in)
  • Quang Tri Nguyen - , National Cancer Institute (NCI) (Autor:in)
  • Sudhir Varma - , National Institute of Allergy and Infectious Diseases (NIAID) (Autor:in)
  • Claudia Dittfeld - , OncoRay - Nationales Zentrum für Strahlenforschung in der Onkologie (Autor:in)
  • Leoni A. Kunz-Schughart - , OncoRay - Nationales Zentrum für Strahlenforschung in der Onkologie (Autor:in)
  • Ralf Kemmerling - , Paracelsus Medizinischen Privatuniversität (Autor:in)
  • Maria R. Becker - , National Cancer Institute (NCI) (Autor:in)
  • Kerstin Heselmeyer-Haddad - , National Cancer Institute (NCI) (Autor:in)
  • Thomas Ried - , National Cancer Institute (NCI) (Autor:in)

Abstract

Colorectal carcinomas (CRC) might be organized hierarchically and contain a subpopulation of tumorigenic, putative cancer stem cells that are CD133 positive. We studied the biological and genetic characteristics of such cells in CRC cell lines and primary tumors. Three CRC cell lines were sorted in CD133 positive and negative fractions. The respective genetic aberration profiles were studied using array comparative genomic hybridization (aCGH) and expression profiling. Tumorigenicity for each cellular population was tested by injection into nude mice. Additionally, we compared CD133+ and CD133- cells of 12 primary colorectal tumors using laser capture microdissection and aCGH. Three of five CRC cell lines displayed both CD133+ and CD133-cells, but tumorigenicity of these subfractions did not differ significantly and aCGH revealed essentially identical genomic imbalances. However, 96 genes were differentially expressed between the two populations. Array comparative genomic hybridization analysis after laser capture microdissection of CD133+ and CD133-areas in primary colorectal tumors revealed genetic differences in 7 of 12 cases. The use of cell lines for studying genomic alterations that define cancer stem cell characteristics, therefore, seems questionable. In contrast, CD133+ cells in primary cancer samples showed a unique genomic aberration profile. In conclusion, our data suggest that CD133 positivity defines a genetically distinct cellular compartment in primary CRC, which potentially includes tumor initiating cells.

Details

OriginalspracheEnglisch
Seiten (von - bis)1478-1488
Seitenumfang11
FachzeitschriftAmerican Journal of Pathology
Jahrgang178
Ausgabenummer4
PublikationsstatusVeröffentlicht - Apr. 2011
Peer-Review-StatusJa

Schlagworte

Ziele für nachhaltige Entwicklung

ASJC Scopus Sachgebiete