Fluorescence correlation spectroscopy
Publikation: Beitrag in Fachzeitschrift › Forschungsartikel › Beigetragen › Begutachtung
Beitragende
Abstract
Fluorescence correlation spectroscopy (FCS) is a powerful technique to measure concentrations, mobilities, and interactions of fluorescent biomolecules. It can be applied to various biological systems such as simple homogeneous solutions, cells, artificial, or cellular membranes and whole organisms. Here, we introduce the basic principle of FCS, discuss its application to biological questions as well as its limitations and challenges, present an overview of novel technical developments to overcome those challenges, and conclude with speculations about the future applications of fluorescence fluctuation spectroscopy.
Details
Originalsprache | Englisch |
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Seiten (von - bis) | 361-368 |
Seitenumfang | 8 |
Fachzeitschrift | BIOESSAYS |
Jahrgang | 34 |
Ausgabenummer | 5 |
Publikationsstatus | Veröffentlicht - Mai 2012 |
Peer-Review-Status | Ja |
Externe IDs
PubMed | 22415816 |
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Schlagworte
ASJC Scopus Sachgebiete
Schlagwörter
- Diffusion, Dynamics, Fluorescence, Fluorescence correlation spectroscopy, Interaction