Expression of the zebrafish recognition molecule F3/F11/contactin in a subset of differentiating neurons is regulated by cofactors associated with LIM domains

Publikation: Beitrag in FachzeitschriftForschungsartikelBeigetragenBegutachtung

Beitragende

  • Dimitrios Gimnopoulos - , Universität Hamburg (Autor:in)
  • Catherina G. Becker - , Universität Hamburg (Autor:in)
  • Heather P. Ostendorff - , Universität Hamburg (Autor:in)
  • Ingolf Bach - , Universität Hamburg (Autor:in)
  • Melitta Schachner - , Universität Hamburg (Autor:in)
  • Thomas Becker - , Universität Hamburg (Autor:in)

Abstract

We have identified a zebrafish homolog of the F3/F11/contactin (F3) recognition molecule. The gene shares 55% amino acid identity with F3 molecules in other vertebrates. Expression of F3 mRNA is first detectable at 16 h post-fertilization (hpf) in trigeminal and Rohon-Beard neurons. At 18-24 hpf, additional weaker expression is present in discrete cell clusters in the hindbrain, in the anterior lateral line/acoustic ganglion and in spinal motor neurons. Transcription factors of the LIM homeodomain class (LIM-HD) and their associated cofactors CLIM/NLI/Ldb (CLIM) have been implicated in the development of peripheral axons of trigeminal and Rohon-Beard neurons. We demonstrate that ectopic overexpression of a dominant-negative CLIM molecule early during zebrafish development strongly reduces expression of F3 mRNA in these neurons indicating regulation of F3 by the LIM-HD protein network. These results and the spatiotemporal correlation of F3 expression with axonal differentiation in a subset of primary neurons suggest an important role of F3 for axon growth.

Details

OriginalspracheEnglisch
Seiten (von - bis)S135-S141
FachzeitschriftMechanisms of Development
Jahrgang119
AusgabenummerSUPPL. 1
PublikationsstatusVeröffentlicht - Dez. 2002
Peer-Review-StatusJa
Extern publiziertJa

Externe IDs

PubMed 14516675

Schlagworte

ASJC Scopus Sachgebiete

Schlagwörter

  • CLIM/NLI/Ldb cofactors, Immunoglobulin- superfamily, Isl-1, L1.1, L1.2, LIM domain, Motor neurons, Rohon-Beard neurons, Trigeminal ganglion