Exploratory study of the prognostic value of microenvironmental parameters during fractionated irradiation in human squamous cell carcinoma xenografts

Publikation: Beitrag in FachzeitschriftForschungsartikelBeigetragenBegutachtung

Beitragende

  • Ala Yaromina - , OncoRay - National Centre for Radiation Research in Oncology (Autor:in)
  • Theresa Kroeber - , OncoRay - National Centre for Radiation Research in Oncology (Autor:in)
  • Andreas Meinzer - , OncoRay - National Centre for Radiation Research in Oncology (Autor:in)
  • Simon Boeke - , OncoRay - National Centre for Radiation Research in Oncology (Autor:in)
  • Howard Thames - , University of Texas Health Science Center at Houston (Autor:in)
  • Michael Baumann - , OncoRay - Nationales Zentrum für Strahlenforschung in der Onkologie, Experimentelles Zentrum an der Medizinischen Fakultät (Autor:in)
  • Daniel Zips - , OncoRay - National Centre for Radiation Research in Oncology (Autor:in)

Abstract

Purpose: To explore the prognostic value of microenvironmental parameters for local tumor control determined before and during fractionated irradiation. Methods and Materials: Six human squamous cell carcinoma (hSCC) lines were transplanted subcutaneously into the right hind leg of nude mice. Tumors were irradiated with 30 fractions within 6 weeks. Local tumor control was determined 120 days after irradiation. Radiation response was quantified as dose to cure 50% of tumors (TCD50). In parallel, untreated and irradiated tumors were excised after injection of pimonidazole (hypoxia marker) and Hoechst 33342 (perfusion marker) for histological evaluation. Results: Pimonidazole hypoxia decreased during fractionated irradiation in the majority of tumor lines. Fraction of perfused vessels and vascular area showed modest changes during fractionated irradiation. Histological parameters before treatment and after three and five fractions did not significantly correlate with TCD50 after irradiation with 30 fractions within 6 weeks (p > 0.05). Hypoxic volume and perfused vessels after 10 fractions showed a significant association with local tumor control after fractionated irradiation (p = 0.018 and p = 0.019, respectively). None of these parameters remained statistically significant when the p value was adjusted for multiple comparisons. Conclusions: The results from this exploratory study suggest that determination of microenvironmental parameters during treatment provides better prognostic information for the outcome after fractionated radiotherapy than pretreatment parameters, which warrants further investigation and confirmation in experimental and clinical studies.

Details

OriginalspracheEnglisch
Seiten (von - bis)1205-1213
Seitenumfang9
FachzeitschriftInternational Journal of Radiation Oncology Biology Physics
Jahrgang80
Ausgabenummer4
PublikationsstatusVeröffentlicht - 15 Juli 2011
Peer-Review-StatusJa

Externe IDs

PubMed 21489709

Schlagworte

Ziele für nachhaltige Entwicklung

Schlagwörter

  • Fractionated radiation, Human tumor xenograft, Local tumor control, Perfusion, Pimonidazole