Equine CRISP-3: Primary structure and expression in the male genital tract

Publikation: Beitrag in FachzeitschriftForschungsartikelBeigetragenBegutachtung

Beitragende

  • A. Schambony - , Stiftung Tierärztliche Hochschule Hannover (TiHo) (Autor:in)
  • M. Gentzel - , Stiftung Tierärztliche Hochschule Hannover (TiHo) (Autor:in)
  • H. Wolfes - , Leibniz Universität Hannover (LUH) (Autor:in)
  • M. Raida - , Pharis Biotec GmbH (Autor:in)
  • U. Neumann - , Stiftung Tierärztliche Hochschule Hannover (TiHo) (Autor:in)
  • E. Töpfer-Petersen - , Stiftung Tierärztliche Hochschule Hannover (TiHo) (Autor:in)

Abstract

Although originally described in the male rodent genital tract, cysteine-rich secretory proteins (CRISPs) are expressed in a variety of mammalian tissue and cell types. The proteins of the male genital tract have been observed associated to spermatozoa and are believed to play a role in mammalian fertilization. Here we describe the identification and primary structure of the first equine member of the CRISP family. Equine CRISP-3 is transcribed and expressed in the stallion salivary gland, in the ampulla and the seminal vesicle. It displays all 16 conserved cysteine residues and shows 82% homology to human and 78% to guinea pig CRISP-2 (AA1, TPX 1) and 77% to human CRISP-3. In contrast to other mammalia, in the horse CRISP-3 is synthesized in great amounts in the accessory sexual glands, ampulla and seminal vesicle, thus allowing the isolation of equine CRISP-3 in amounts suitable for biochemical, physiological and structural studies from stallion seminal plasma. Copyright (C) 1998 Elsevier Science B.V.

Details

OriginalspracheEnglisch
Seiten (von - bis)206-216
Seitenumfang11
FachzeitschriftBiochimica et Biophysica Acta - Protein Structure and Molecular Enzymology
Jahrgang1387
Ausgabenummer1-2
PublikationsstatusVeröffentlicht - 8 Sept. 1998
Peer-Review-StatusJa
Extern publiziertJa

Externe IDs

PubMed 9748582
ORCID /0000-0002-4482-6010/work/142251055

Schlagworte

Schlagwörter

  • cDNA, Cysteine-rich secretory protein, Expression, Liquid chromatography-mass spectrometry, Seminal plasma, Stallion

Bibliotheksschlagworte