Endosteal and Perivascular Subniches in a 3D Bone Marrow Model for Multiple Myeloma

Publikation: Beitrag in FachzeitschriftForschungsartikelBeigetragenBegutachtung

Beitragende

Abstract

The bone marrow microenvironment is the preferred location of multiple myeloma, supporting tumor growth and development. It is composed of a collection of interacting subniches, including the endosteal and perivascular niche. Current in vitro models mimic either of these subniches. By developing a model combining both niches, this study aims to further enhance the ability to culture primary myeloma cells in vitro. Also, the dependency of myeloma cells on each niche was studied. A 3D bone marrow model containing two subniches was created using 3D bioprinting technology. We used a bioprintable pasty calcium phosphate cement (CPC) scaffold with seeded osteogenic multipotent mesenchymal stromal cells (O-MSCs) to model the endosteal niche, and Matrigel containing both endothelial progenitor cells (EPCs) and MSCs to model the perivascular niche. Within the model containing one or both of the niches, primary CD138+ myeloma cells were cultured and analyzed for both survival and proliferation. The 3D bone marrow model with combined subniches significantly increasing the proliferation of CD138+ myeloma cells compared to both environments separately. The developed model showed an essential role of the perivascular niche over the endosteal niche in supporting myeloma cells. The developed model can be used to study the expansion of primary myeloma cells and their interactions with varying bone marrow subniches.

Details

OriginalspracheEnglisch
Seiten (von - bis)300-312
Seitenumfang13
FachzeitschriftTissue Engineering - Part C: Methods
Jahrgang24
Ausgabenummer5
PublikationsstatusVeröffentlicht - Mai 2018
Peer-Review-StatusJa

Externe IDs

PubMed 29652626
ORCID /0000-0001-9075-5121/work/160047996

Schlagworte

Schlagwörter

  • 3D culture model, bioprinting, bone marrow niche, multiple myeloma, tumor microenvironment