Embryonic stem cells as an in vitro model for mutagenicity, cytotoxicity and embryotoxicity studies: Present state and future prospects

Publikation: Beitrag in FachzeitschriftKurze Umfrage/ÜbersichtsartikelBeigetragenBegutachtung

Beitragende

  • J. Rohwedel - , Universität zu Lübeck (Autor:in)
  • K. Guan - , Institut für Pharmakologie und Toxikologie, Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung (Autor:in)
  • C. Hegert - , Universität zu Lübeck (Autor:in)
  • A. M. Wobus - , Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung (Autor:in)

Abstract

Primary cultures or established cell lines of vertebrates are commonly used to analyse the mutagenic, embryotoxic or teratogenic potential of environmental factors, drugs and xenobiotics in vitro. However, these cellular systems do not include developmental processes from early embryonic stages up to terminally differentiated cell types. An alternative approach has been offered by permanent lines of pluripotent stem cells of embryonic origin, such as embryonic carcinoma (EC), embryonic stem (ES) and embryonic germ (EG) cells. The undifferentiated stem cell lines are characterized by nearly unlimited self-renewal capacity and have been shown to differentiate in vitro into cells of all three primary germ layers. Pluripotent embryonic stem cell lines recapitulate cellular developmental processes and gene expression patterns of early embryogenesis during in vitro differentiation, data which are summarized in this review. In addition, recent studies are presented which investigated mutagenic, cytotoxic and embryotoxic effects of chemical substances using in vitro systems of pluripotent embryonic stem cells. Furthermore, an outlook is given on future molecular technologies using embryonic stem cells in developmental toxicology and embryotoxicology.

Details

OriginalspracheEnglisch
Seiten (von - bis)741-753
Seitenumfang13
FachzeitschriftToxicology in vitro : official journal of the European Society of Toxicology in Vitro
Jahrgang15
Ausgabenummer6
PublikationsstatusVeröffentlicht - 2001
Peer-Review-StatusJa

Externe IDs

PubMed 11698176

Schlagworte

ASJC Scopus Sachgebiete

Schlagwörter

  • Cytotoxicity, Differentiation, Embryonic stem cells, Embryotoxicity, Gene expression patterns, Mouse, Mutagenicity